Upotreba izoliranog monomera lignina iz crnog luga dobivenoga od praznih grozdova palmina ploda kao prebiotika

IF 0.7 4区农林科学 Q4 MATERIALS SCIENCE, PAPER & WOOD

Drvna Industrija Pub Date : 2023-03-25 DOI:10.5552/drvind.2023.0015

S. Hidayati, Subeki Subeki, M. M. Harahap, S. Hadi

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Abstract

Waste is generated in the form of black liquor during pulp processing. Furthermore, lignin, the main ingredient of black liquor, contains a phenylpropanoid compound with prebiotic and antimicrobial activity. Also, it is a component of lignocellulose that has prebiotic and antimicrobial activity effects due to its indigestible nature and it comprises phenylpropanoid components. Therefore, this study examines and identifies the lignin purification and test results of its prebiotic activity. The technique used to identify lignin fraction is called Thin Layer Chromatography (TLC) and Gas Chromatography-Mass Spectrometry (GC-MS) plates. Prebiotic activity tests were performed using the calculation of total bacteria on the growth of Lactobacillus casei. The results showed that the purification process using CHCl3, 3 % MeOH:CHCl3, 20 % MeOH:CHCl3, and MeOH yielded 10.68 %, 6.34 %, 11.38 % 44.85 %, respectively. The 3 % MeOH:CHCl3 fraction contained benzaldehyde, 4- hydroxy-3,5- dimethoxy, 1-methylbutyl hexadecanoate, oleic acid, and di-2-ethylhexyl phthalate. The 3 % MeOH:CHCl3 fractionate with a concentration of 15 % also showed a prebiotic activity for L. casei at 6.1 x 102 colonies/mL.

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使用从空棕榈树中获得的黑空气中分离的木材单体作为益生元

在纸浆加工过程中，废物以黑液的形式产生。此外，黑液的主要成分木质素含有一种具有益生元和抗菌活性的苯丙烷类化合物。此外，它是木质纤维素的一种成分，由于其不易消化的性质而具有益生元和抗菌活性，并且它包括苯丙烷类成分。因此，本研究对木质素的纯化及其益生元活性的测试结果进行了检验和鉴定。用于鉴定木质素组分的技术被称为薄层色谱法（TLC）和气相色谱-质谱法（GC-MS）板。使用对干酪乳杆菌生长的总细菌的计算进行益生元活性测试。结果表明，使用CHCl3、3%MeOH:CHCl3、20%MeOH:CCHl3和MeOH的纯化过程分别产生10.68%、6.34%、11.38%和44.85%。3%MeOH:CHCl3馏分含有苯甲醛、4-羟基-3,5-二甲氧基、十六烷酸1-甲基丁酯、油酸和邻苯二甲酸二-2-乙基己酯。浓度为15%的3%MeOH:CHCl3分馏物对干酪乳杆菌也显示出6.1 x 102菌落/mL的益生元活性。

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来源期刊

Drvna Industrija MATERIALS SCIENCE, PAPER & WOOD-

CiteScore

1.80

自引率

9.10%

发文量

审稿时长

>12 weeks

期刊介绍： "Drvna industrija" ("Wood Industry") journal publishes original scientific and review papers, short notes, professional papers, conference papers, reports, professional information, bibliographical and survey articles and general notes relating to the forestry exploitation, biology, chemistry, physics and technology of wood, pulp and paper and wood components, including production, management and marketing aspects in the woodworking industry.