Total Flavonoids Determination and Antioxidant Activity of Ethyl Acetate, Ethanol, and Methanol Extracts from Seluang Belum Root (Luvunga sarmentosa (Blume) Kurz.)

Berkala Kedokteran Pub Date : 2023-02-28 DOI:10.20527/jbk.v19i1.15733

N. Wathan, M. Rizki, Amalia Khairunnisa, Hilneser Simamora

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Abstract

Abstract: Flavonoids are phenolic compounds from nature that found in almost all parts of the plants. Seluang belum (Luvunga sarmentosa (Blume) Kurz.) root is one of the medicinal plants used by Dayak people in Central and South Kalimantan where in previous studies its part was contains flavonoids. This study aims to evaluate the best solvent for extracting flavonoid content and antioxidant activity using ethanol, methanol and ethyl acetate. The antioxidant activity performed by in vitro technique by DPPH assay. Total flavonoids content on the extracts of L. sarmentosa root determined using UV-Vis spectrophotometric with aluminium chloride method and calculated as quercetin equivalents (QE). The results showed the highest antioxidant activities confirmed for the methanol extract (IC50 = 80.33 ppm). The highest of total flavonoids contents was recorded for the ethanol extract (6.56 ± 0.006% w/w Quercetin Equivalent). The results showed that treatment effect of different solvent extraction was significant (sig <0.05) on total flavonoid and antioxidant activities from L. sarmentosa root.

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紫藤乙酸乙酯、乙醇、甲醇提取物总黄酮含量测定及抗氧化活性研究

摘要：黄酮类化合物是自然界中的酚类化合物，几乎存在于植物的所有部位。Seluang belum（Luvunga sarmentosa（Blume）Kurz）根是中加里曼丹和南加里曼丹达亚克人使用的药用植物之一，在以前的研究中，其部分含有黄酮类化合物。本研究旨在评价乙醇、甲醇和乙酸乙酯提取黄酮含量和抗氧化活性的最佳溶剂。DPPH法测定体外抗氧化活性。采用紫外-可见分光光度法和氯化铝法测定了酸根提取物中总黄酮的含量，并以槲皮素当量（QE）计算。结果表明，甲醇提取物的抗氧化活性最高（IC50=80.33ppm）。乙醇提取物的总黄酮含量最高（6.56±0.006%w/w槲皮素当量）。结果表明，不同溶剂萃取处理对酸根总黄酮和抗氧化活性有显著影响（sig<0.05）。

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Berkala Kedokteran

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24 weeks