The Effect of High-Intensity Interval Training on the Level of Peroxisome proliferator-activated receptor gamma and PR domain containing 16 Proteins in Adipose Tissue in Overweight Type 2 Diabetic Male Sprague-Dawley Rats with Diabetes

Abstract

Introduction: PPAR-γ and PRDM16 proteins are key proteins in the adipose tissue regulation and the white tissue conversion to the brown and beige adipose tissue. Type 2 diabetes can interfere with the function of these two proteins and, functionally, leads to reduced activity of these proteins. The role of exercise on these two important proteins has not been studied yet in subcutaneous adipose tissue. The purpose of the present study was to investigate the effect of high-intensity interval training (HIIT) on the level of PPAR-γ and PRDM16 proteins in adipose tissue in overweight type 2 diabetic male Sprague-Dawley rats with diabetes. Materials & Methods: In this study, 16 two-month-old Sprague-Dawley rats with a mean weight of 270±20 g were selected. After diabetic induction with STZ and Nicotinamide, rats were randomly assigned to two groups, training (8 heads) and control (8 heads). The training group trained for 4 days a week in accordance with the training program for 8 weeks, while the control group did not have any training program. The independent t-test was used to analyze the data of PPAR-γ and PRDM16 proteins. Results: There was a significant rise in PRDM16 protein content (p<0.001) in the training group compared to control, though this rise was not meaningful in PPAR-γ protein content (p=0.26). Conclusions: HIIT training has led to a significant increase in the content of the PRDM16 protein, which plays a vital role in the conversion of white to brown fat cells. Hence, it is expected that a new insight into the root, differentiation, and preservation of the white adipose tissue and its conversion into brown adipose tissue will be achieved.