Enhancing cell survival in 3D printing of organoids using innovative bioinks loaded with pre-cellularized porous microscaffolds

Q1 Computer Science

Bioprinting Pub Date : 2022-12-01 DOI:10.1016/j.bprint.2022.e00247

Adrien Rousselle , Arielle Ferrandon , Eric Mathieu , Julien Godet , Vincent Ball , Leo Comperat , Hugo Oliveira , Philippe Lavalle , Dominique Vautier , Youri Arntz

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引用次数: 1

Abstract

Extrusion bioprinting is a relevant 3D technology to create biological systems in regenerative medicine, pharmaceutical development and cancer research. Bioink is the necessary component to incorporate the cells that will be printed by extrusion bioprinting. However, bioinks and extrusion printing can generate shear stresses mechanically unfavorable for cell survival. We thus developed a bioink, based on methacrylated collagen and hyaluronic acid, in combination with porous poly(D,L-lactic-co-glycolic acid) solid microscaffolds to protect cells against mechanical stress during extrusion printing. We found that porosities of the microscaffolds allowed human chondosarcoma cells to colonize the structure. Moreover, metabolic activity of these chondrosarcoma cells, fibroblast cells, and dental pulp stem cells (DPSCs) incorporated within bioink (before printing) increased 4-fold in presence of a polylysine- or collagen-coated microscaffolds compared with those cultured without microscaffolds. Their survival increased by 10% either by hand deposition or by bioprinting extrusion (bioprinter BioBot®Basic) compared to cells in bioink without microscaffolds. In addition to the mechanoshield properties provided by microscaffolds, they allow the migration of DPSCs stem cells towards HCS-2/8 cancer cells after 7 days of co-culture in an organoid created by bioprinting extrusion while without microscaffolds the cells aggregated and remained static.

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使用载有预细胞化多孔微支架的创新生物墨水提高类器官3D打印中的细胞存活率

挤出生物打印是一种相关的3D技术，用于在再生医学、药物开发和癌症研究中创建生物系统。生物墨水是必要的组成部分，以纳入细胞，将被挤出生物打印。然而，生物油墨和挤压印刷会产生对细胞存活不利的机械剪切应力。因此，我们开发了一种基于甲基丙烯酸化胶原蛋白和透明质酸的生物墨水，结合多孔聚(D, l -乳酸-羟基乙酸)固体微支架，以保护细胞免受挤压打印过程中的机械应力。我们发现微支架的多孔性允许人类软骨肉瘤细胞在结构上定植。此外，这些软骨肉瘤细胞、成纤维细胞和牙髓干细胞(DPSCs)在生物墨水中(打印前)加入聚赖氨酸或胶原包被的微支架后，其代谢活性比没有微支架培养的细胞增加了4倍。与没有微支架的生物墨水中的细胞相比，通过手工沉积或生物打印挤出(生物打印机BioBot®Basic)，它们的存活率提高了10%。除了微支架提供的机械屏蔽特性外，在生物打印挤压产生的类器官中共培养7天后，它们允许DPSCs干细胞向HCS-2/8癌细胞迁移，而没有微支架的细胞聚集并保持静止。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioprinting Computer Science-Computer Science Applications

CiteScore

11.50

自引率

0.00%

发文量

审稿时长

68 days

期刊介绍： Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.