Development of In vitro Mass Propagation Protocol for Gerbera (Gerbera jamesonii Bolus) var. Orange

N. Akter, Selim Sarkar, Naimul Hasan, S. Rahman, Md Abdur Rauf Sarkar
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引用次数: 1

Abstract

An in vitro mass propagation protocol was developed for Gerbera (Gerbera jamesonii Bolus) var. orange using flower bud explants. The young (7 days) explants were found to be suitable for culture and highest (100%) callus induction was obtained in MS medium supplemented with 5.0 mg/l BAP with 1.0 mg/l NAA. The maximum shoot regeneration (95%) and the number of shoots (10) were observed in MS medium supplemented with 6.0 mg/l BAP with 0.3 mg/l NAA. The highest shoot multiplication frequency (95%) and the number of shoots (60) were obtained in MS medium supplemented with only 2.0 mg/l BAP. The well-developed highest number of roots (5.0) per plant and average root length (5.0 cm) with 100% root induction efficiency without callusing was recorded in the half strength of MS medium devoid of any hormonal supplement. Acclimatized in vitro grown plantlets were successfully transferred into the field condition. Plant Tissue Cult. & Biotech. 32(2): 217-226, 2022 (December)
桔非洲菊(Gerbera jamesonii Bolus)品种离体大批量繁殖方案的开发
采用花蕾外植体对非洲菊(Gerbera jamesonii Bolus)变种橙进行了体外大规模繁殖。幼(7天)外植体适合培养,在添加5.0mg/l BAP和1.0mg/l NAA的MS培养基中获得最高(100%)的愈伤组织诱导。在添加6.0mg/l BAP和0.3mg/l NAA的MS培养基中观察到最大的芽再生率(95%)和芽数(10)。在只添加2.0mg/l BAP的MS培养基中获得了最高的芽增殖频率(95%)和芽数(60)。在没有任何激素补充的半强度MS培养基中,记录到每株植物发育良好的最高根数(5.0)和平均根长(5.0cm),具有100%的无愈伤组织的根诱导效率。经过驯化的试管苗被成功地转移到田间条件下。植物组织培养生物技术。32(2):217-2262022(12月)
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