A two-minute assay for electronic quantification of antibodies in saliva enabled through a reusable microfluidic multi-frequency impedance cytometer and machine learning analysis

Abstract

The use of saliva as a diagnostic fluid has always been appealing due to the ability for rapid and non-invasive sampling for monitoring health status and the onset and progression of disease and treatment progress. Saliva is rich in protein biomarkers and provides a wealth of information for diagnosis and prognosis of various disease conditions. Portable electronic tools which rapidly monitor protein biomarkers would facilitate point-of-care diagnosis and monitoring of various health conditions. For example, the detection of antibodies in saliva can enable rapid diagnosis and tracking disease pathogenesis of various auto-immune diseases like sepsis. Here, we present a novel method involving immuno-capture of proteins on antibody coated beads and electrical detection of dielectric properties of the beads. The changes in electrical properties of a bead when capturing proteins are extremely complex and difficult to model physically in an accurate manner. The ability to measure impedance of thousands of beads at multiple frequencies, however, allows for a data-driven approach for protein quantification. By moving from a physics driven approach to a data driven approach, we have developed, for the first time ever to the best of our knowledge, an electronic assay using a reusable microfluidic impedance cytometer chip in conjunction with supervised machine learning to quantifying immunoglobulins G (IgG) and immunoglobulins A (IgA) in saliva within two minutes.