Phytogenic Synthesis and Characterization of Silver Metallic/Bimetallic Nanoparticles Using Beta vulgaris L. Extract and Assessments of Their Potential Biological Activities

IF 2.5 4区综合性期刊 Q2 CHEMISTRY, MULTIDISCIPLINARY

Applied Sciences-Basel Pub Date : 2023-09-08 DOI:10.3390/app131810110

Khaled M. Elattar, A. A. Ghoniem, Fatimah Al-Otibi, Mohammed S. El-Hersh, Yosra A. Helmy, W. Saber

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引用次数: 1

Abstract

The synthesis of novel nanomedicines through eco-friendly protocols has been applied on a large scale with the prediction of discovering alternate therapies. The current work attained phytogenic synthesis of Ag-mNPs, AgSeO2-bmNPs, and Ag-TiO2-bmNPs through bio-reduction using an aqueous extract of Beta vulgaris (red beetroot). The phytochemical profile of the eco-friendly synthesized metallic/bimetallic nanoparticles was studied. The optical properties of nano-solutions were studied via UV-visible spectroscopy. The Fourier-transform infrared spectroscopy (FT-IR) spectral analyses revealed that stretching vibrations at wavenumbers 3303.81–3327.81 cm−1 attributed to phenolic hydroxyl groups documented shifts in the values in this range owing to proton dissociation through the bio-reduction of the metal ions. The surface morphology and the charge of the nanoparticles were investigated using a Transmission Electron Microscope (TEM) and zeta potential analyses. The prepared nano-solutions showed lower antioxidant activity (1,1-Diphenyl-2-picrylhydrazyl (DPPH•) and phosphomolybdate assays) than the plant extract. These results together with phytochemical analyses support the participation of the reactive species (phenolic contents) in the bio-reduction of the metal ions in the solutions through the formation of metallic/bimetallic nanoparticles. Ag-mNPs, AgSeO2-bmNPs, and Ag-TiO2-bmNPs showed antibacterial potentiality. AgSeO2-bmNPs were superior with inhibitory zone diameters of 34.7, 37.7, 11.7, and 32.7 mm against Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, and Salmonella enterica, respectively. Applying the Methylthiazole Tetrazolium (MTT) assay, the Ag-TiO2 bmNPs revealed potent cytotoxicity against the HePG2 tumor cell line (IC50 = 18.18 ± 1.5 µg/mL), while Ag-SeO2 bmNPs revealed the most potent cytotoxicity against the MCF-7 cell line (IC50 = 17.92 ± 1.4 µg/mL).

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用甜菜提取物合成金属银/双金属纳米粒子及其潜在生物活性评价

通过生态友好协议合成的新型纳米药物已被大规模应用，并有望发现替代疗法。目前的工作通过生物还原利用甜菜根(红甜菜根)的水提取物获得了Ag-mNPs、AgSeO2-bmNPs和Ag-TiO2-bmNPs的植物性合成。研究了生态友好型合成金属/双金属纳米颗粒的植物化学性质。通过紫外可见光谱研究了纳米溶液的光学性质。傅里叶变换红外光谱(FT-IR)光谱分析表明，在3303.81-3327.81 cm−1的波数范围内，由于金属离子的生物还原导致质子解离，由酚羟基引起的拉伸振动记录了该范围内数值的变化。利用透射电子显微镜(TEM)和zeta电位分析研究了纳米颗粒的表面形貌和电荷。制备的纳米溶液的抗氧化活性(1,1-二苯基-2-苦酰肼(DPPH•)和磷酸钼酸盐测定)低于植物提取物。这些结果与植物化学分析一起支持活性物质(酚含量)通过形成金属/双金属纳米颗粒参与溶液中金属离子的生物还原。Ag-mNPs、AgSeO2-bmNPs和Ag-TiO2-bmNPs表现出抗菌潜力。AgSeO2-bmNPs对粪肠球菌、金黄色葡萄球菌、大肠杆菌和肠炎沙门氏菌的抑菌带直径分别为34.7、37.7、11.7和32.7 mm。甲基噻唑四唑(MTT)检测表明，Ag-TiO2 bmNPs对HePG2肿瘤细胞系的IC50值为18.18±1.5µg/mL, Ag-SeO2 bmNPs对MCF-7细胞系的IC50值为17.92±1.4µg/mL。

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来源期刊

Applied Sciences-Basel CHEMISTRY, MULTIDISCIPLINARYMATERIALS SCIE-MATERIALS SCIENCE, MULTIDISCIPLINARY

CiteScore

5.30

自引率

11.10%

发文量

10882

期刊介绍： Applied Sciences (ISSN 2076-3417) provides an advanced forum on all aspects of applied natural sciences. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.