Effect of Reduced Chemical Fertilizers with Biochar Application on Microbial Community in Rhizosphere Soil of Amorphophallus konjac

IF 0.5 4区医学

Journal of Biobased Materials and Bioenergy Pub Date : 2023-04-01 DOI:10.1166/jbmb.2023.2258

Yongzhong Hong, Z. Fan, Sheng-guang Xu, Chunmei Pan, Xiaohua Zhang, Li Lin, Jinwen Zhang

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Abstract

In agricultural applications, biochar showed potentials to improve soil properties. At present, effects of chemical fertilizer reduction with biochar application on the microbial community in the rhizosphere soil need to be further investigated. In this study, konjac (Amorphophallus konjac K. Koch) was taken as the research subject, and analyzing the microbial α-diversity, amplicon sequence variants (ASVs) community composition in rhizosphere were analyzed using high-throughput sequencing technology, and tuber yield was calculated. Four treatments of chemical fertilizer with biochar application were set, T1: common fertilizer 20 g/plant, T2: common fertilizer 10 g/plant, T3: controlled-release fertilizers 10 g/plant, T4: controlled-release fertilizers 10 g/plant with biochar 100 g/plant. The results found that, the Chao1 index, number of unique ASVs, and total ASV of rhizosphere soil bacteria in T2 increased by 14.45, 13.39, and 14.36% respectively, compared to T1; while that of fungal abundance decreased by 17.82, 48.28, and 18.42%. Similarly, compared to T3, the Chao1 index, number of unique ASVs, and total ASV in bacterial of rhizosphere soil in T4 increased by 9.36, 19.88, and 9.81%, respectively, while that of fungal abundance decreased by 9.16, 20.18, and 9.62%. The yield of konjac showed no significant difference between T1 and T2 treatments. However, T4 treatment had a significantly higher yield than T3 treatment (P = 0.076), and T4 treatment was significantly higher than T1 treatment (P < 0.05). The results indicated that chemical fertilizer reduction with biochar application can improve bacterial abundance and unique and total ASVs in the rhizosphere soil of konjac, while reducing fungal abundance and unique and total ASVs. This can effectively promote the transformation of the rhizosphere soil towards a high-fertilizer “bacterial type”, leading to an increase in the yield of konjac.

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还本施用生物炭对魔芋根际土壤微生物群落的影响

在农业应用中，生物炭显示出改善土壤性质的潜力。目前，生物炭减施化肥对根际土壤微生物群落的影响有待进一步研究。本研究以魔芋(Amorphophallus konjac K. Koch)为研究对象，利用高通量测序技术对魔芋根际微生物α-多样性、扩增子序列变异(amplicon sequence variation, asv)群落组成进行分析，并计算块茎产量。生物炭配施化肥4个处理，T1:普通肥20 g/株，T2:普通肥10 g/株，T3:控释肥10 g/株，T4:控释肥10 g/株，生物炭100 g/株。结果发现，T2处理根际土壤细菌的Chao1指数、独特ASV数和总ASV数分别比T1提高了14.45%、13.39%和14.36%;真菌丰度分别下降了17.82%、48.28%和18.42%。同样，与T3相比，T4根际土壤的Chao1指数、独特ASV数量和细菌总数分别增加了9.36%、19.88%和9.81%，真菌丰度分别下降了9.16%、20.18%和9.62%。魔芋产量在T1和T2处理间无显著差异。但T4处理产量显著高于T3处理(P = 0.076)， T4处理产量显著高于T1处理(P < 0.05)。结果表明，施用生物炭减肥可提高魔芋根际土壤细菌丰度、独特asv和总asv，降低真菌丰度、独特asv和总asv。这样可以有效地促进根际土壤向高肥“细菌型”转化，从而提高魔芋产量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biobased Materials and Bioenergy 工程技术-材料科学：生物材料

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0.00%

发文量

审稿时长

6 months