A muscadine locus confers resistance to predominant species of grapevine root-knot nematodes (Meloidogyne spp.) including virulent populations

IF 2.2 3区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

OENO One Pub Date : 2023-08-31 DOI:10.20870/oeno-one.2023.57.3.7443

D. Esmenjaud, M. Banora, Ulysse Julien-Portier, Maria-Das-Dores Lafargue, J. Tandonnet, Bernadette Rubio, Andrew Walker, C. Van Ghelder, Nathalie Ollat

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引用次数: 0

Abstract

Root-knot nematodes (RKNs) Meloidogyne spp. are extremely polyphagous pests and four species severely affect grapevines throughout the world: M. arenaria, M. incognita, M. javanica and M. ethiopica. Californian populations of M. arenaria and M. incognita are reported to be virulent to widely used rootstocks and to the rootstock ‘Harmony’ in particular. Breeding RKNs-resistant grape rootstocks is a promising alternative to highly toxic nematicides. Muscadine (Vitis rotundifolia syn. Muscadinia rotundifolia) is a resistance (R) source with undercharacterised genetics. To this end, we used a segregating progeny between the RKN-resistant Vitis x Muscadinia accession ‘VRH8771’ from the muscadine source ‘NC184-4’ and the RKN-susceptible V. vinifera cv. Cabernet-Sauvignon. We first phenotyped its resistance to isolates of the i) M. arenaria, ii) M. incognita and iii) M. javanica species, and then to iv) two mixed Harmony-virulent Californian populations of M. arenaria and M. incognita. Finally, we created an isolate of M. arenaria and M. incognita from these Harmony populations and phenotyped the progeny to each of them [v) and vi)], and to vii) an isolate of M. ethiopica. The resistance phenotype of all the progeny’s individuals was independent of the RKN isolates or populations used. Resistance was mapped in a region of chromosome 18 in VRH8771, supporting the hypothesis that it is conferred by a single gene with an unprecedented wide spectrum in grapevine, including Harmony-virulent isolates. This dominant gene, referred to as MsppR1, is linked to the telomeric QTL XiR4 for X. index resistance from the same source. Additionally, plant mortality data showed that MsppR1-resistant material expressed a high-level resistance to the Harmony-virulent isolates. Our results are a first step towards the development of marker-assisted breeding using SSR and SNP markers for resistance to RKNs in accession VRH8771.

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麝香碱位点对葡萄根结线虫(Meloidogyne属)的优势种(包括毒性种群)具有抗性

根结线虫（RKNs）根结线虫属（Meloidogyne spp。培育抗RKNs的葡萄砧木是一种很有前途的高毒杀线虫剂的替代品。麝香碱（Vitis rotundifolia syn.Muscadinia rotundiforia）是一种遗传特征不明确的抗性（R）来源。为此，我们使用了来自毒蕈碱来源“NC184-4”的抗RKN葡萄x毒蕈碱登录号“VRH8771”和对RKN敏感的葡萄品种赤霞珠之间的分离后代。我们首先对其对i）M.arenaria、ii）M.incognita和iii）M.javanica物种的分离株的抗性进行了表型分析，然后对iv）两个混合的Harmony毒力加州种群M.arenarria和M.incognata进行了抗性分型。最后，我们从这些Harmony群体中创建了一个M.arenaria和M.incognita的分离株，并对它们的后代进行了表型分析[v）和vi）]，以及vii）一个M.ethiopica分离株。所有后代个体的抗性表型与所使用的RKN分离株或群体无关。抗性被定位在VRH8771的18号染色体区域，支持了一种假说，即它是由葡萄中具有前所未有的广谱性的单一基因赋予的，包括Harmony毒力分离株。该显性基因被称为MsppR1，与来自同一来源的X.指数抗性的端粒QTL XiR4相连。此外，植物死亡率数据显示，MsppR1抗性材料对Harmony毒力分离株表现出高水平的抗性。我们的结果是利用SSR和SNP标记在登录号VRH8771中开发抗RKN的标记辅助育种的第一步。

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来源期刊

OENO One Agricultural and Biological Sciences-Food Science

CiteScore

4.40

自引率

13.80%

发文量

审稿时长

13 weeks

期刊介绍： OENO One is a peer-reviewed journal that publishes original research, reviews, mini-reviews, short communications, perspectives and spotlights in the areas of viticulture, grapevine physiology, genomics and genetics, oenology, winemaking technology and processes, wine chemistry and quality, analytical chemistry, microbiology, sensory and consumer sciences, safety and health. OENO One belongs to the International Viticulture and Enology Society - IVES, an academic association dedicated to viticulture and enology.