Development of gold, silver, and iron nanoparticles using isolated berberine: its characterization and antimicrobial and cytotoxic activities against COLO320DM and Hela cells

IF 2.2 4区工程技术 Q2 Chemistry

Gold Bulletin Pub Date : 2022-02-18 DOI:10.1007/s13404-022-00309-9

Mangesh A. Bhutkar, Dheeraj S. Randive, Somnath D. Bhinge, Ganesh H. Wadkar, Sachin S. Todkar, Kiran P. Shejawal

{"title":"Development of gold, silver, and iron nanoparticles using isolated berberine: its characterization and antimicrobial and cytotoxic activities against COLO320DM and Hela cells","authors":"Mangesh A. Bhutkar, Dheeraj S. Randive, Somnath D. Bhinge, Ganesh H. Wadkar, Sachin S. Todkar, Kiran P. Shejawal","doi":"10.1007/s13404-022-00309-9","DOIUrl":null,"url":null,"abstract":"<div><h2>Abstract\n</h2><div><p>The current report discloses the development of gold, silver, and iron nanoparticles using berberine by green synthesis and its evaluation for antimicrobial activity and in vitro cytotoxicity against colon cancer and human cervical cancer cells. The prepared nanoparticles were characterized by Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction analysis. The characterization of results of the prepared nanoparticles satisfied the distinctive properties of the potent nanodrug. The antimicrobial potential was exhibited by all the prepared nanoparticles against the selected microbial strains. The zone of inhibition (mm) exhibited by gold nanoparticles biosynthesized using berberine (BAuNPs) was 15.66 ± 0.47 mm against <i>Pseudomonas aeruginosa</i>, 15.0 ± 1.41 against <i>Escherichia coli</i>, and 15.33 ± 1.24 mm against <i>Staphylococcus aureus</i>. The cytotoxicity study exhibited promising results as compared to pure berberine. The results of the MTT assay of gold nanoparticles against COLO320DM exhibited 92.27 ± 0.14% inhibition, whereas in the case of the SRB assay, it revealed 89.72 ± 0.29% inhibition as compared to other nanoparticles and pure berberine. For Hela cell lines, in the case of the MTT assay, BAuNPs exhibited 92.13 ± 0.64% inhibition while in the SRB assay, it revealed 85.56 ± 0.39% inhibition. The results of the Trypan blue assay revealed that BAuNPs showed 76.54 ± 1.00% inhibition against the COLO320DM cell line, whereas in the case of Hela cell lines, 78.03 ± 1.47% inhibition was observed. The effect exhibited by BAuNPs may be attributed to increased production of reactive oxygen species by treated carcinoma cells that results in oxidation of vital constituents which ultimately causes death of the cell. Thus, these in vitro results demonstrate the promising therapeutic applications of the BAuNPs in nanomedicine.</p></div></div>","PeriodicalId":55086,"journal":{"name":"Gold Bulletin","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gold Bulletin","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s13404-022-00309-9","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Chemistry","Score":null,"Total":0}

引用次数: 4

Abstract

The current report discloses the development of gold, silver, and iron nanoparticles using berberine by green synthesis and its evaluation for antimicrobial activity and in vitro cytotoxicity against colon cancer and human cervical cancer cells. The prepared nanoparticles were characterized by Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction analysis. The characterization of results of the prepared nanoparticles satisfied the distinctive properties of the potent nanodrug. The antimicrobial potential was exhibited by all the prepared nanoparticles against the selected microbial strains. The zone of inhibition (mm) exhibited by gold nanoparticles biosynthesized using berberine (BAuNPs) was 15.66 ± 0.47 mm against Pseudomonas aeruginosa, 15.0 ± 1.41 against Escherichia coli, and 15.33 ± 1.24 mm against Staphylococcus aureus. The cytotoxicity study exhibited promising results as compared to pure berberine. The results of the MTT assay of gold nanoparticles against COLO320DM exhibited 92.27 ± 0.14% inhibition, whereas in the case of the SRB assay, it revealed 89.72 ± 0.29% inhibition as compared to other nanoparticles and pure berberine. For Hela cell lines, in the case of the MTT assay, BAuNPs exhibited 92.13 ± 0.64% inhibition while in the SRB assay, it revealed 85.56 ± 0.39% inhibition. The results of the Trypan blue assay revealed that BAuNPs showed 76.54 ± 1.00% inhibition against the COLO320DM cell line, whereas in the case of Hela cell lines, 78.03 ± 1.47% inhibition was observed. The effect exhibited by BAuNPs may be attributed to increased production of reactive oxygen species by treated carcinoma cells that results in oxidation of vital constituents which ultimately causes death of the cell. Thus, these in vitro results demonstrate the promising therapeutic applications of the BAuNPs in nanomedicine.

查看原文本刊更多论文

利用分离的小檗碱制备金、银和铁纳米颗粒:其表征及其对COLO320DM和Hela细胞的抗菌和细胞毒性活性

摘要本文介绍了利用小檗碱绿色合成金、银、铁纳米粒子的研究进展及其对结肠癌和人宫颈癌细胞的抑菌活性和体外细胞毒性评价。采用傅里叶变换红外光谱(FT-IR)、扫描电子显微镜(SEM)、透射电子显微镜(TEM)和x射线衍射分析对制备的纳米颗粒进行了表征。制备的纳米颗粒的表征结果符合强效纳米药物的独特性质。所制备的纳米颗粒对所选的微生物菌株均表现出抗菌潜力。小檗碱合成的金纳米颗粒对铜绿假单胞菌的抑制区(mm)为15.66±0.47 mm，对大肠杆菌的抑制区为15.0±1.41 mm，对金黄色葡萄球菌的抑制区为15.33±1.24 mm。与纯小檗碱相比，细胞毒性研究显示出有希望的结果。在MTT实验中，金纳米颗粒对COLO320DM的抑制率为92.27±0.14%，而在SRB实验中，与其他纳米颗粒和纯小檗碱相比，金纳米颗粒的抑制率为89.72±0.29%。对于Hela细胞系，在MTT试验中，BAuNPs的抑制率为92.13±0.64%，而在SRB试验中，BAuNPs的抑制率为85.56±0.39%。台普兰蓝实验结果显示，BAuNPs对COLO320DM细胞株的抑制作用为76.54±1.00%，而对Hela细胞株的抑制作用为78.03±1.47%。BAuNPs所表现出的效果可能归因于经处理的癌细胞活性氧的产生增加，导致重要成分氧化，最终导致细胞死亡。因此，这些体外实验结果证明了BAuNPs在纳米医学中的治疗应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Gold Bulletin 工程技术-材料科学：综合

CiteScore

3.30

自引率

4.50%

发文量

审稿时长

3 months

期刊介绍： Gold Bulletin is the premier international peer reviewed journal on the latest science, technology and applications of gold. It includes papers on the latest research advances, state-of-the-art reviews, conference reports, book reviews and highlights of patents and scientific literature. Gold Bulletin does not publish manuscripts covering the snthesis of Gold nanoparticles in the presence of plant extracts or other nature-derived extracts. Gold Bulletin has been published over 40 years as a multidisciplinary journal read by chemists, physicists, engineers, metallurgists, materials scientists, biotechnologists, surface scientists, and nanotechnologists amongst others, both within industry and academia. Gold Bulletin is published in Association with the World Gold Council.