Cell migration of preosteoblast cells on a clinical gelatin sponge for 3D bone tissue engineering

IF 3.9 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Chi-Yun Wang, Zong-Keng Kuo, M. Hsieh, L. Ke, Chihchen Chen, Chao-Min Cheng, P. Lai
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引用次数: 6

Abstract

Using three-dimensional (3D) bone engineering to fabricate bone segments is a better choice for repairing bone defects than using autologous bone. However, biomaterials for bone engineering are burdened with some clinical safety concerns. In this study, we layered commonly found clinical materials, hemostatic gelatin sponges, in a novel manner to create a 3D scaffold for bone engineering purposes. We further examined the comparable benefits of our design with both closed- and open-bottom holders. Cells in stacked layer disc systems were examined after a week of growth and differentiation. Osteoblasts in the outer layers of both closed- and open-bottom holder systems displayed gradually increased alkaline phosphatase (ALP) activity but decreased osteopontin (OPN) expression. Further, cell proliferation assays and LIVE/DEAD staining revealed decreased viable cell counts in the top layer with increased incubation time. However, while layered disc systems with closed-bottom holders underwent differentiation, they kept more differentiated cells alive within the gelatin sponge disc scaffold after 28 d of culturing. Whether cells were inoculated into the top, middle, or bottom portions of the layered disc stack, osteoblasts showed a preference for migrating to the top layer, in keeping with the oxygen and nutrients gradients. Regarding practical application, this study offers valuable information to promote the use of hemostatic gelatin sponges for bone engineering.
成骨前细胞在三维骨组织工程明胶海绵上的细胞迁移
采用三维骨工程技术制备骨段是修复骨缺损较好的选择。然而,用于骨工程的生物材料在临床应用中存在一定的安全性问题。在这项研究中,我们分层常见的临床材料,止血明胶海绵,以一种新颖的方式来创建用于骨工程目的的3D支架。我们进一步研究了我们的设计与封闭式和开放式底部持有人的可比效益。细胞在堆叠层盘系统中生长和分化一周后进行检查。封闭底支架和开底支架外层的成骨细胞碱性磷酸酶(ALP)活性逐渐升高,骨桥蛋白(OPN)表达逐渐降低。此外,细胞增殖试验和LIVE/DEAD染色显示,随着孵育时间的增加,顶层的活细胞计数减少。然而,尽管具有封闭底支架的层状盘系统进行了分化,但在培养28 d后,它们在明胶海绵盘支架内保留了更多的分化细胞。无论将细胞接种到层状椎间盘堆的顶部、中间还是底部,成骨细胞都倾向于迁移到顶层,以保持氧气和营养梯度。在实际应用方面,本研究为促进止血明胶海绵在骨工程中的应用提供了有价值的信息。
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来源期刊
Biomedical materials
Biomedical materials 工程技术-材料科学:生物材料
CiteScore
6.70
自引率
7.50%
发文量
294
审稿时长
3 months
期刊介绍: The goal of the journal is to publish original research findings and critical reviews that contribute to our knowledge about the composition, properties, and performance of materials for all applications relevant to human healthcare. Typical areas of interest include (but are not limited to): -Synthesis/characterization of biomedical materials- Nature-inspired synthesis/biomineralization of biomedical materials- In vitro/in vivo performance of biomedical materials- Biofabrication technologies/applications: 3D bioprinting, bioink development, bioassembly & biopatterning- Microfluidic systems (including disease models): fabrication, testing & translational applications- Tissue engineering/regenerative medicine- Interaction of molecules/cells with materials- Effects of biomaterials on stem cell behaviour- Growth factors/genes/cells incorporated into biomedical materials- Biophysical cues/biocompatibility pathways in biomedical materials performance- Clinical applications of biomedical materials for cell therapies in disease (cancer etc)- Nanomedicine, nanotoxicology and nanopathology- Pharmacokinetic considerations in drug delivery systems- Risks of contrast media in imaging systems- Biosafety aspects of gene delivery agents- Preclinical and clinical performance of implantable biomedical materials- Translational and regulatory matters
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