Optimization of solvent-free microwave-assisted extraction of antioxidant compounds from Lagenaria siceraria fruit by response surface methodology

IF 2.2 4区农林科学 Q2 HORTICULTURE

Folia Horticulturae Pub Date : 2022-08-19 DOI:10.2478/fhort-2022-0013

Sahar Iftikhar, Muhammad Tariq Qamar, A. Y. Aydar, D. Ahmed

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引用次数: 2

Abstract

Abstract Lagenaria siceraria (bottle gourd) fruit is highly celebrated for its nutritional as well as therapeutic properties. The present study was undertaken with the aim of exploring an efficient green solvent-free microwave-assisted extraction method for bioactives from this fruit. Optimization was done according to response surface methodology (RSM), where microwave power (W) and time (s) were independent factors, and percent extraction yield, total phenolic content (TPC), total flavonoid content (TFC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant potential (FRAP) and iron chelating activity (ICA) were the responses. TPC, TFC, DPPH radical scavenging activity, FRAP and ICA were highest at 480 W and 60 s. The TPC was 288.9 mg GAE · g−1 DW (milligram gallic acid equivalent per gram dry weight), TFC was 214.1 mg RE · g−1 (rutin equivalent per gram DW), anti-radical activity was 32.96%, FRAP was 289.7 mg AAE · g−1 (mg ascorbic acid equivalents per gram) and ICA was 19.52%. The results of the study thus demonstrate that the solvent-free microwave-assisted extraction method, which utilised an optimum power of 480 W and a time of 60 s, is an effective and green method for extraction of antioxidant compounds from bottle gourd fruit.

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响应面法优化无溶剂微波辅助提取西瓜果实抗氧化化合物

摘要冬瓜（Lagenaria siceraria）果实因其营养和治疗特性而备受赞誉。本研究旨在探索一种有效的绿色无溶剂微波辅助提取该果实生物活性物质的方法。根据响应面法（RSM）进行优化，其中微波功率（W）和时间（s）是独立因素，提取率、总酚含量（TPC）、总黄酮含量（TFC）、1，1-二苯基-2-苦基肼（DPPH）自由基清除活性、铁还原抗氧化潜力（FRAP）和铁螯合活性（ICA）是响应。TPC、TFC、DPPH自由基清除活性、FRAP和ICA在480 W和60 s时最高。TPC为288.9 mg GAE·g−1 DW（毫克没食子酸当量/克干重），TFC为214.1 mg RE·g−2（芦丁当量/克DW），抗自由基活性为32.96%，FRAP为289.7mg AAE·g−1（mg抗坏血酸当量/克），ICA为19.52%。研究结果表明，无溶剂微波辅助提取法是一种从葫芦果实中提取抗氧化化合物的有效且绿色的方法，最佳功率为480W，时间为60s。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Folia Horticulturae Agricultural and Biological Sciences-Horticulture

CiteScore

3.40

自引率

0.00%

发文量

审稿时长

16 weeks

期刊介绍： Folia Horticulturae is an international, scientific journal published in English. It covers a broad research spectrum of aspects related to horticultural science that are of interest to a wide scientific community and have an impact on progress in both basic and applied research carried out with the use of horticultural crops and their products. The journal’s aim is to disseminate recent findings and serve as a forum for presenting views as well as for discussing important problems and prospects of modern horticulture, particularly in relation to sustainable production of high yield and quality of horticultural products, including their impact on human health.