Mapping of leaf rust resistance genes in common wheat ‘Guinong08-6’

IF 0.4 4区农林科学 Q4 AGRICULTURE, MULTIDISCIPLINARY

Semina-ciencias Agrarias Pub Date : 2023-05-03 DOI:10.5433/1679-0359.2023v44n2p601

Luhua Li, Jianshu Sui, Mingjian Ren, Ruhong Xu

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引用次数: 0

Abstract

Wheat leaf rust (Puccinia triticina Eriks.), a devastating disease of wheat in the world, causes severe yield losses and therefore the development of resistant cultivars is very important. Here, a Chinese wheat line (Guinong08-6) showed adult-plant resistance against mixed fungal isolates of leaf rust, which is common in Guiyang region. It was crossed with a susceptible wheat line (Guinong19) to develop F1, F2, and F3 hybrids. Combined SSR and STS markers were used to map leaf rust resistance genes in Guinong08-6, and the resistance phenotype of Guinong08-6 was co-regulated by two complementary dominant genes, named LrGn08-6A and LrGn08-6B. LrGn08-6A was mapped to chromosome 2AS with markers URIC-LN2 and Xgpw2204, which flanked the gene at distances of 1.8 centimorgan (cM) and 14.83 cM, respectively. LrGn08-6B was mapped to chromosome 4DL with markers Xgpw342 and Xbarc93, which both flanked the gene at a distance of 26.57 cM. Genetic and molecular marker analyses demonstrated that LrGn08-6A, which was inherited from Aegilops ventricosa may be the resistance gene Lr37, while LrGn08-6B may be a newly discovered leaf rust resistance gene.

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普通小麦“桂农08-6”抗叶锈病基因定位

小麦叶锈病（Puccinia triticina Eriks。）是世界上一种破坏性的小麦病害，造成严重的产量损失，因此开发抗病品种至关重要。在贵阳地区常见的叶锈病混合真菌菌株中，中国小麦品系桂农08-6表现出成株抗性。将其与感病小麦品系（桂农19）杂交，形成F1、F2和F3杂交种。利用SSR和STS标记对桂农08-6的叶锈病抗性基因进行了定位，桂农08-8的抗性表型由两个互补的显性基因LrGn08-6A和LrGn08.6B共同调控。用标记URIC-LN2和Xgpw2204将LrGn08-6A定位到染色体2AS上，这两个标记分别位于基因两侧1.8厘米（cM）和14.83厘米。用标记Xgpw342和Xbarc93将LrGn08-6B定位到4DL染色体上，这两个标记都位于基因两侧26.57cM处。遗传和分子标记分析表明，从脑室山羊草遗传的LrGn08.6A可能是抗性基因Lr37，而LrGn08 6B可能是新发现的叶锈抗性基因。

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来源期刊

Semina-ciencias Agrarias 农林科学-农业综合

CiteScore

1.10

自引率

0.00%

发文量

148

审稿时长

3-6 weeks

期刊介绍： The Journal Semina Ciencias Agrarias (Semina: Cien. Agrar.) is a quarterly publication promoting Science and Technology and is associated with the State University of Londrina. It publishes original and review articles, as well as case reports and communications in the field of Agricultural Sciences, Animal Sciences, Food Sciences and Veterinary Medicine.