First Report on Molecular Diagnosis and Phylogenetic Analysis of Hepatozoon canis in Naturally Infected Domesticated and Stray Dogs from Jhang, Pakistan

Q2 Agricultural and Biological Sciences
M. Abbas
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Abstract

Canine diseases, particularly caused by tick-borne hepatozoons are responsible for high morbidity and mortality and are the reason for attracting significant focus. The current study was aimed to survey the occurrence of H. canis in domesticated and stray dogs of Jhang city (Punjab) with its molecular identification. For this, blood samples from 300 dogs (n=200 domesticated; n=100 stray) were collected and assayed through PCR for the detection of H. canis supported by sequencing analysis. The results demonstrated, 15.66% (47/300) of samples positive for H. canis. A significantly (P<0.05) higher occurrence of H. canis was observed in stray dogs (27%) than domesticated dogs (10%). Evaluation of the various risk factors showed that the age, sex, breed, dog category (domestic or stray), body coat, environmental settings (rural or urban) and ectoparasitic infestation were significantly (P < 0.05) associated with the occurrence of infection. The phylogenetic analysis of the PCR confirmed specimens revealed a very close homology of the detected strains with the ones diagnosed earlier in China and Malta. It was concluded that present moderate prevalence of H. canis among the dog populations in the area of Jhang may rise with stray dogs being most vulnerable hosts and potential source of vectors spread. © 2021 Friends Science Publishers
巴基斯坦Jhang自然感染驯养狗和流浪狗犬肝人畜共患病分子诊断和系统发育分析的首次报告
犬类疾病,特别是由蜱传肝动物引起的疾病,是高发病率和高死亡率的原因,也是引起人们关注的原因。本研究旨在通过分子鉴定调查Jhang市(旁遮普邦)驯养狗和流浪狗中犬H.canis的发生情况。为此,收集了300只狗(n=200只驯养狗;n=100只流浪狗)的血液样本,并通过PCR进行分析,以检测犬H。结果表明,15.66%(47/300)的样本对犬H。流浪狗(27%)犬H.canis的发生率显著高于家养狗(10%)(P<0.05)。对各种危险因素的评估表明,年龄、性别、品种、犬类(家养或流浪)、体毛、环境(农村或城市)和体外寄生虫感染与感染的发生显著相关(P<0.05)。PCR确认标本的系统发育分析显示,检测到的菌株与中国和马耳他早期诊断出的菌株具有非常密切的同源性。得出的结论是,目前Jhang地区狗群中犬H.canis的中度流行率可能会上升,流浪狗是最脆弱的宿主,也是媒介传播的潜在来源。©2021 Friends Science出版社
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来源期刊
International Journal of Agriculture and Biology
International Journal of Agriculture and Biology AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
1.70
自引率
0.00%
发文量
40
审稿时长
5 months
期刊介绍: Information not localized
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