High-frequency Shoot Multiplication and In Vitro Plantlet Regeneration from Shoot Bud Explants in Curcuma zedoaria Roscoe

IF 1.2 Q4 PHARMACOLOGY & PHARMACY

Journal of Herbs, Spices and Medicinal Plants Pub Date : 2022-11-07 DOI:10.1080/10496475.2022.2143463

Shajaat Hussain, Nisha Kapoor, R. Mahajan

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Abstract

ABSTRACT A rapid and efficient micropropagation protocol was developed for Curcuma zedoaria, using shoot buds as explants. Explants established on basal MS medium were transferred to 12 different medium compositions with different combinations and concentrations of plant growth hormones 6-benzylaminopurine (BAP), kinetin (Kn), and thidiazuron (TDZ). The best shoot multiplication was recorded in MS medium supplemented with BAP (2.0 mg L−1) and TDZ (1.5 mg L−1), resulting in 5.3 ± 0.24 shoots of per explant and shoot length of 6.12 ± 0.19 cm. For the best in vitro root induction, the aseptic shoots excised from the culture flasks and inoculated individually on MS medium supplemented with 1.5 mg L−1 indole-3-butyric acid (IBA) resulted in 74.2% root growth and 8.82 ± 0.31 roots per explant. Although callus induction was observed in MS containing 2,4-dichlorophenoxyacetic acid (2, 4-D) and 1-naphthaleneacetic acid (NAA), the highest callus proliferation was observed with MS and NAA (2.0 mg L−1). Micropropagated plants established on soil showed 83% survival on acclimatization to the field conditions.

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莪术嫩芽高频增殖及离体植株再生研究

摘要以莪术(Curcuma zedoaria)茎芽为外植体，研究了莪术快速高效的微繁技术。将建立在MS基础培养基上的外植体转移到12种不同的培养基中，这些培养基中含有不同组合和浓度的植物生长激素6-苄基氨基嘌呤(BAP)、动蛋白(Kn)和硫代氮脲(TDZ)。在添加BAP (2.0 mg L−1)和TDZ (1.5 mg L−1)的MS培养基中，芽增殖效果最好，每个外植体的芽数为5.3±0.24个，芽长为6.12±0.19 cm。从培养瓶中取出无菌苗，单独接种于添加1.5 mg L−1吲哚-3-丁酸(IBA)的MS培养基上，其离体生根率为74.2%，每个外植体生根率为8.82±0.31根。虽然在含有2,4-二氯苯氧乙酸(2,4-d)和1-萘乙酸(NAA)的培养基中可以诱导愈伤组织，但在MS和NAA (2.0 mg L−1)的培养基中愈伤组织增殖能力最强。在土壤上培养的小苗对田间环境的适应成活率为83%。

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来源期刊

Journal of Herbs, Spices and Medicinal Plants Medicine-Complementary and Alternative Medicine

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.