Spectrofluorimetric Determination of Some N. Containing Medicines Using Rhodamine 6G as a Chromogenic Reagent

Abstract

A sensitive spectrofluorimetric method has been developed for the analysis of some medicines containing primary, secondary, and tertiary amino groups, namely Diclofenac (DIC), Domperidone (DOM), Famotidine (FAM), and Propranolol (PRO), in their pure and medicinal forms. The method is based on the quenching of the fluorescence intensity of rhodamine 6G (R6G) through the formation of ion-pair complexes between the above medicines and the R-6G reagent, which is measured at 552 nm after excitation at 402 nm. The calibration graphs were rectilinear in the concentration ranges of 0.10- 9.00, 0.05-15.00, 0.10-14.0 and 0.05-5.00 µg mL-1 for above medicines respectively. The recovery (%) values were ranged between 99.45%- 100.97%. The detection limits ranged in the concentration of 0.243-0.754 µg/mL, and the limits of quantitation were 0.806- 2.420 µgmL-1 for all drugs. The method was successfully applied for the determination of these drugs in their pharmaceutical preparations.