Phospholipid-based nanodrill technology for enhanced intracellular delivery of nano-sized cargos

Q1 Engineering
Doyeon Kim , Seung Soo Nam , Hyunbum Jeon , Youngheun Cho , Eunji Sim , Hyuncheol Kim
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引用次数: 0

Abstract

Nanosized drug delivery systems typically enter the cell via endocytosis. However, a significant amount of the endocytosed cargo cannot effectively escape from the endosome, resulting in drug degradation. Therefore, there are several ongoing efforts to develop transmembrane delivery systems that could circumvent endocytosis. In this study, phospholipid nanotube nanodrills (LDs) were formed onto the surface of a human serum albumin nanoparticle via self-assembling phospholipids. The nanodrill technology enhanced the intracellular uptake efficiency of nanoparticles via energy-independent direct cell membrane permeation. The length of the nanodrills according to the DSPE-PEG to DSPC ratio was investigated both experimentally and theoretically. Our findings demonstrated that longer nanodrills were formed on the surface of the nanoparticles as the ratio of DSPC (i.e., a strongly hydrophobic lipid) in the two phospholipids increases. Moreover, the intracellular uptake efficiency increased as the length of phospholipid nanodrills increased. In addition to enhancing intracellular delivery, the phospholipid nanodrills could penetrate the extracellular matrix and enable the introduction of nanoparticles, thus highlighting the promising tissue penetration capacity of phospholipid nanodrill technology. The improved cell permeability of LD technology was demonstrated by effectively inhibiting specific genes via siRNA-based therapeutic delivery. Moreover, this approach enhanced the efficacy of chemotherapeutics against chemo-resistant cancer cells. Therefore, LD technology could be used to deliver genetic materials and chemical-based therapeutics both in vitro and in vivo.

Abstract Image

基于磷脂的纳米钻技术用于增强纳米级货物的细胞内递送
纳米级药物输送系统通常通过内吞作用进入细胞。然而,大量的内吞货物不能有效地从内核体中逃脱,导致药物降解。因此,有几个正在进行的努力,以开发跨膜传递系统,可以绕过内吞作用。本研究通过磷脂的自组装,在人血清白蛋白纳米颗粒表面形成磷脂纳米管纳米钻(ld)。纳米钻技术通过不依赖能量的直接细胞膜渗透提高了纳米颗粒在细胞内的摄取效率。实验和理论研究了DSPE-PEG与DSPC比对纳米钻长度的影响。我们的研究结果表明,随着两种磷脂中dsc(即一种强疏水脂质)的比例增加,纳米颗粒表面形成了更长的纳米钻。此外,细胞内摄取效率随磷脂纳米钻长度的增加而增加。除了增强细胞内递送外,磷脂纳米钻还可以穿透细胞外基质,从而使纳米颗粒的引入成为可能,从而突出了磷脂纳米钻技术有前途的组织渗透能力。通过基于sirna的治疗递送,LD技术可以有效抑制特定基因,从而提高细胞通透性。此外,这种方法提高了化疗药物对化疗耐药癌细胞的疗效。因此,LD技术可用于体外和体内传递遗传物质和化学疗法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Smart Materials in Medicine
Smart Materials in Medicine Engineering-Biomedical Engineering
CiteScore
14.00
自引率
0.00%
发文量
41
审稿时长
48 days
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