Antioxidant and Anti-proliferative Activities of Smilax Zeylanica Root and Rhizome Extract against Liver Carcinoma Cells

IF 1.2 Q4 PHARMACOLOGY & PHARMACY

Journal of Herbs, Spices and Medicinal Plants Pub Date : 2021-04-03 DOI:10.1080/10496475.2021.1891178

N. Malge, A.M.P.W. Bandara, W. Keerthirathna, D. Dissanayake, Patharage Kamal Perera, C. Witharana, L. Peiris

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引用次数: 1

Abstract

ABSTRACT The antioxidant, cytotoxic, and anti-cancer potential of aqueous root and rhizome extract (ARRE) of Smilax zeylanica was investigated against human liver carcinoma cells. The phytochemical composition was evaluated by standard methods, and confirmed by thin-layer chromatography. Free radical scavenging activity was determined by DPPH assay, cytotoxic activity by brine shrimp lethality assay, and anti-cancer potential was investigated using liver carcinoma HepG2 cells. Phytochemical analysis revealed the presence of saponins, flavonoids, proanthocyanins, tannins, and polyphenols. The total phenolic and flavonoid content varied from 0.28 to 5.26 mg gallic acid equivalent 100 mg−1 and 0.25–50.26 quercetin equivalent 100 mg−1, respectively. There was a linear relationship between the free radical scavenging activity and phytochemical concentrations with an IC50 value of 108 ± 1.2 µg mL−1 compared to the standard (IC50: 2.59 ± 0.02 µg mL−1). The brine shrimp lethality assay showed strong cell killing ability (IC50: 84 ± 0.7 µg mL−1) and the extract induced apoptosis of HepG2 cells (77 ± 1.52%) at 300 µg mL−1, comparable to the positive control (camptothecin).

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泽兰根和根茎提取物对肝癌细胞的抗氧化和抗增殖活性

摘要研究了泽兰的根状茎水提取物（ARRE）对人肝癌细胞的抗氧化、细胞毒性和抗癌作用。通过标准方法评估植物化学成分，并通过薄层色谱法确认。通过DPPH测定法测定自由基清除活性，通过盐水虾致死率测定法测定细胞毒性活性，并用肝癌HepG2细胞研究抗癌潜力。植物化学分析显示存在皂苷、类黄酮、原花青素、单宁和多酚。总酚类和类黄酮含量在0.28至5.26 mg之间没食子酸当量100 mg−1和槲皮素当量0.25–50.26，分别为100 mg−1。自由基清除活性与植物化学物质浓度之间存在线性关系，与标准品相比，IC50值为108±1.2µg mL−1（IC50:2.59±0.02µg mL-1）。盐水虾致死率测定显示出较强的细胞杀伤能力（IC50:84±0.7µg mL−1），提取物在300µg mL–1时诱导HepG2细胞凋亡（77±1.52%），与阳性对照（喜树碱）相当。

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来源期刊

Journal of Herbs, Spices and Medicinal Plants Medicine-Complementary and Alternative Medicine

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.