{"title":"Comparative RNA sequencing for deciphering nodes of multiple abiotic stress tolerance in lentil (Lens culinaris Medikus)","authors":"Dharmendra Singh , Jyoti Taunk , Chandan Kumar Singh , Priya Chaudhary , Kishor Gaikwad , Rajendra Kumar Yadav , Deepti Singh , Madan Pal","doi":"10.1016/j.plgene.2022.100373","DOIUrl":null,"url":null,"abstract":"<div><p><span><span><span><span>Lentil is a highly proteinaceous crop whose productivity is severely hampered by various abiotic stresses. </span>Transcriptome analysis unveils important metabolic pathways, </span>regulatory genes<span><span> and can help in identification of genomic resources which can be utilized for ameliorating abiotic stress tolerance. Therefore, the present study was undertaken to dissect transcriptomes of contrasting lentil genotypes under different stresses; drought, heat, salinity and alkalinity. Differential gene expression analysis and annotation were conducted by de novo </span>transcriptomics<span> and their functional correlation under diverse stresses was established using gene ontology analyses. Major differentially expressed genes (DEGs) which were found to be up-regulated under drought and heat stresses were involved in organic acid synthesis and </span></span></span>translational regulation. Similarly, DEGs which were co-upregulated under salinity and alkalinity stresses belonged to </span>abscisic acid<span><span><span><span> (ABA) signaling, epigenetic regulation, </span>sucrose transport, vesicular trafficking and regulation of </span>cation transport at the root symplast-xylem interface. Data generated in this study can be used to elucidate converging tolerance pathways under multiple abiotic stresses. The information can be utilized in </span>molecular breeding<span> and gene pyramiding programmes of lentil and related species.</span></span></p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Gene","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352407322000233","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 5
Abstract
Lentil is a highly proteinaceous crop whose productivity is severely hampered by various abiotic stresses. Transcriptome analysis unveils important metabolic pathways, regulatory genes and can help in identification of genomic resources which can be utilized for ameliorating abiotic stress tolerance. Therefore, the present study was undertaken to dissect transcriptomes of contrasting lentil genotypes under different stresses; drought, heat, salinity and alkalinity. Differential gene expression analysis and annotation were conducted by de novo transcriptomics and their functional correlation under diverse stresses was established using gene ontology analyses. Major differentially expressed genes (DEGs) which were found to be up-regulated under drought and heat stresses were involved in organic acid synthesis and translational regulation. Similarly, DEGs which were co-upregulated under salinity and alkalinity stresses belonged to abscisic acid (ABA) signaling, epigenetic regulation, sucrose transport, vesicular trafficking and regulation of cation transport at the root symplast-xylem interface. Data generated in this study can be used to elucidate converging tolerance pathways under multiple abiotic stresses. The information can be utilized in molecular breeding and gene pyramiding programmes of lentil and related species.
Plant GeneAgricultural and Biological Sciences-Plant Science
CiteScore
4.50
自引率
0.00%
发文量
42
审稿时长
51 days
期刊介绍:
Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.