The effects of lithium on human red blood cells studied using optical spectroscopy and laser trap

IF 2.2 4区生物学 Q3 BIOPHYSICS

European Biophysics Journal Pub Date : 2023-03-17 DOI:10.1007/s00249-023-01643-2

Yashveer Singh, Aniket Chowdhury, Raktim Dasgupta, Shovan Kumar Majumder

{"title":"The effects of lithium on human red blood cells studied using optical spectroscopy and laser trap","authors":"Yashveer Singh, Aniket Chowdhury, Raktim Dasgupta, Shovan Kumar Majumder","doi":"10.1007/s00249-023-01643-2","DOIUrl":null,"url":null,"abstract":"<div><p>\nLithium has been the treatment of choice for patients with bipolar disorder<b>.</b> However, lithium overdose happens more frequently since it has a very narrow therapeutic range in blood, necessitating investigation of its adverse effects on blood cells. The possible changes that lithium exposure may have on functional and morphological characteristics of human red blood cells (RBCs) have been studied ex vivo using single-cell Raman spectroscopy, optical trapping, and membrane fluorescent probe. The Raman spectroscopy was performed with excitation at 532 nm light, which also results in simultaneous photoreduction of intracellular hemoglobin (Hb). The level of photoreduction of lithium-exposed RBCs was observed to decline with lithium concentration, indicating irreversible oxygenation of intracellular Hb from lithium exposure. The lithium exposure may also have an effect on RBC membrane, which was investigated via optical stretching in a laser trap and the results suggest lower membrane fluidity for the lithium-exposed RBCs. The membrane fluidity of RBCs was further studied using the Prodan generalized polarization method and the results verify the reduction of membrane fluidity upon lithium exposure.</p></div>","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":"52 1-2","pages":"91 - 100"},"PeriodicalIF":2.2000,"publicationDate":"2023-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00249-023-01643-2.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Biophysics Journal","FirstCategoryId":"2","ListUrlMain":"https://link.springer.com/article/10.1007/s00249-023-01643-2","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOPHYSICS","Score":null,"Total":0}

引用次数: 0

Abstract

Lithium has been the treatment of choice for patients with bipolar disorder. However, lithium overdose happens more frequently since it has a very narrow therapeutic range in blood, necessitating investigation of its adverse effects on blood cells. The possible changes that lithium exposure may have on functional and morphological characteristics of human red blood cells (RBCs) have been studied ex vivo using single-cell Raman spectroscopy, optical trapping, and membrane fluorescent probe. The Raman spectroscopy was performed with excitation at 532 nm light, which also results in simultaneous photoreduction of intracellular hemoglobin (Hb). The level of photoreduction of lithium-exposed RBCs was observed to decline with lithium concentration, indicating irreversible oxygenation of intracellular Hb from lithium exposure. The lithium exposure may also have an effect on RBC membrane, which was investigated via optical stretching in a laser trap and the results suggest lower membrane fluidity for the lithium-exposed RBCs. The membrane fluidity of RBCs was further studied using the Prodan generalized polarization method and the results verify the reduction of membrane fluidity upon lithium exposure.

Abstract Image

查看原文本刊更多论文

利用光谱学和激光陷阱研究了锂对人体红细胞的影响

锂一直是双相情感障碍患者的治疗选择。然而，由于锂在血液中的治疗范围非常窄，因此过量发生的频率更高，因此有必要研究其对血细胞的不良影响。利用单细胞拉曼光谱、光捕获和膜荧光探针研究了锂暴露对人红细胞(rbc)功能和形态特征可能产生的变化。在532 nm光激发下进行拉曼光谱，这也导致细胞内血红蛋白(Hb)的同时光还原。观察到锂暴露红细胞的光还原水平随锂浓度下降，表明锂暴露导致细胞内Hb不可逆氧化。锂暴露也可能对红细胞膜产生影响，通过激光陷阱中的光学拉伸研究了这一点，结果表明锂暴露的红细胞膜流动性较低。利用Prodan广义极化法进一步研究了红细胞的膜流动性，结果证实了锂暴露后膜流动性的降低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

European Biophysics Journal 生物-生物物理

CiteScore

4.30

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal publishes papers in the field of biophysics, which is defined as the study of biological phenomena by using physical methods and concepts. Original papers, reviews and Biophysics letters are published. The primary goal of this journal is to advance the understanding of biological structure and function by application of the principles of physical science, and by presenting the work in a biophysical context. Papers employing a distinctively biophysical approach at all levels of biological organisation will be considered, as will both experimental and theoretical studies. The criteria for acceptance are scientific content, originality and relevance to biological systems of current interest and importance. Principal areas of interest include: - Structure and dynamics of biological macromolecules - Membrane biophysics and ion channels - Cell biophysics and organisation - Macromolecular assemblies - Biophysical methods and instrumentation - Advanced microscopics - System dynamics.