The Effect of Green Tea Leaf Extract on Spatial Memory Function and Superoxyde Dismutase Enzyme Activity in Mice with D-galactose Induced Dimentia

Sains Medika Pub Date : 2017-04-04 DOI:10.26532/SAINSMED.V8I1.1050

A. Gumay, Saekhol Bakri, Astika Widy Utomo

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引用次数: 1

Abstract

Background:�Oxidative stress�and�inflammation�play�an important role in�pathogenesis of�brain aging�and neurodegenerative diseases such as�Alzheimer. Green tea�has been shown to have antioxidant, anti-inflammatory, anticancer, and neuroprotective�activity. Objectives: to determine the effect of green tea extract on�spatial memory function and superoxide dismutase�enzyme activity in mice with D-galactose induced dementiaMethods:�An experimental study using "post test only control group design".�Twenty male�BALB/c Mice aged 6-8�weeks were divided into�4�groups.�Negative control group�(NG)�was induced by subcutaneous injection of�D-galactose�(150�mg/kg BW)�once�daily for�6�weeks.�GT-90, GT-270,�GT-540�were induced by�D-galactose�and orally administered with 90, 270, and 540 mg/kg BW of�green tea extract�once daily for 6 weeks.�The spatial memory functions were assessed using Morris water maze and�SOD enzyme activities�were evaluated using ELISA.�One-way Anova and Kruskal-Wallis were used for statistical analysis.�Results: mean�percentage of latency time in the GT-90�(35.29�(SD=�2.69)%),�GT-270�(35.28 (SD= 2.62)%), and�GT-540�(35.62�(SD=5.05)%)�were�significantly�higher compared to that of NG�(20.38�(SD =�3.21)%), p�<0.05). SOD enzyme activity in the�GT-270�(0.78 (SD = 0.07) U/ml)�was�significantly�higher�compared to that of NG�(0.51 (SD = 0.01) U�ml), p= 0.004).Conclusion:�Green tea extract�may�improve�spatial memory�function�and�the activity of�superoxide dismutase�enzyme in mice�with D-galactose induced�dementia.

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绿茶提取物对d -半乳糖致痴呆小鼠空间记忆功能及超氧歧化酶活性的影响

背景：�氧化应激�和�发炎�玩�在�发病机制�大脑老化�以及神经退行性疾病，如�阿尔茨海默病。绿茶�已被证明具有抗氧化、抗炎、抗癌和神经保护作用�活动目的：测定绿茶提取物对�空间记忆功能与超氧化物歧化酶�D-半乳糖诱导的痴呆小鼠的酶活性方法：�一项使用“仅测试后对照组设计”的实验研究。�二十男�6-8岁BALB/c小鼠�周分为�4.�小组。�阴性对照组�（NG）�通过皮下注射�D-半乳糖�（150�mg/kg体重）�一旦�每日�6.�周。�GT-90、GT-270，�GT-540�由�D-半乳糖�并口服90、270和540 mg/kg BW的�绿茶提取物�每天一次，持续6周。�使用Morris水迷宫和�SOD酶活性�使用ELISA进行评估。�Anova和Kruskal-Wallis被用于统计分析。�结果：平均值�GT-90中延迟时间的百分比�（35.29�（SD=�2.69）%），�GT-270�（35.28（SD=2.62）%）�GT-540�（35.62�（SD=5.05）%）�是�显著地�与天然气相比更高�（20.38�（SD=�3.21）%），p�<0.05）�GT-270�（0.78（标准差=0.07）U/ml）�是�显著地�更高�与天然气相比�（0.51（SD=0.01）U�ml），p=0.004）。结论：�绿茶提取物�也许�改进�空间存储器�作用�和�的活动�超氧化物歧化酶�小鼠体内的酶�D-半乳糖诱导�痴呆症。

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Sains Medika

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4 weeks