In vivo study of cardioprotective effect of bay leaf (Syzygium polyanthum) extract

Medisains Pub Date : 2022-04-30 DOI:10.30595/medisains.v20i1.12502

Refi Ikhtiari, Laura Kartika Sari, A. N. Nasution, C. Ginting, Erwin Handoko, I. Lister

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Abstract

Background: The excessive use of chemotherapy drugs such as doxorubicin might induce cardiovascular diseases. Heart-specific biomarker enzymes such as LDH and BNP indicated the early signs of cardiotoxicity. However, there is no report on the effect of bay leaf on LDH and BNP nor its bioactive compounds and histopathology analysis.Objective: This study aims to determine the cardioprotective effect of Ethanol Extract of Bay Leaf (EEBL) based on phytochemical analysis, LDH and BNP levels, and cardiac histology of rats administered with doxorubicin.Methods: About 24 Wistar rats were divided into six groups treated with 100 mg/kg BW, 300 mg/kg BW, and 500 mg/kg BW of EEBL with CMC-Na 0.5 %, vitamin E, and doxorubicin. Preconditioning was 14 days, followed by 21 days of intraperitoneal administration. LDH and BNP parameters were measured on days 1st, 7th, 14th, and 20th. Histology analysis was conducted on day 21st.Results: Dark green EEBL showed sufficient physical quality and properties for practical application in pharmacy. EEBL has significantly affected LDH, BNP, and cardio recovery dose-dependent. The most effective doses were observed at 500 mg/kg BW, and its performance is not statistically different from those of the commercial vitamin E. The histopathological images revealed significant improvements in interstitial edema, wavy fibers, hemorrhage, intracellular vacuole, and inflammatory cell infiltration. Here we report six bioactive compounds obtained from EEBL have cardioprotective effects; Neophytadiene, squalane, phytol, methyl palmitate, stigmasterol, and 9,12-Octadecanoic acid methyl ester.Conclusion: This study has shown the promising potential of bay leaf extract as a cardioprotector with sufficient quality pharmacological standards.

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月桂叶提取物心脏保护作用的体内研究

背景：化疗药物如阿霉素的过量使用可能诱发心血管疾病。心脏特异性生物标志物酶如LDH和BNP表明心脏毒性的早期迹象。然而，目前还没有关于月桂叶对LDH和BNP的影响的报道，也没有关于其生物活性化合物和组织病理学分析的报道。目的：本研究旨在通过植物化学分析、LDH和BNP水平以及阿霉素给药大鼠的心脏组织学，来确定月桂叶乙醇提取物（EEBL）的心脏保护作用。方法：将约24只Wistar大鼠分为6组，分别用100 mg/kg、300 mg/kg和500 mg/kg的EEBL加0.5%羧甲基纤维素钠、维生素E和阿霉素处理。预处理为14天，然后腹膜内给药21天。在第1天、第7天、第14天和第20天测量LDH和BNP参数。第21天进行组织学分析。结果：深绿色EEBL具有足够的物理质量和性能，可在药学上实际应用。EEBL显著影响LDH、BNP和心功能恢复的剂量依赖性。观察到最有效的剂量是500 mg/kg BW，其性能与商业维生素E没有统计学差异。组织病理学图像显示间质水肿、波状纤维、出血、细胞内液泡和炎症细胞浸润有显著改善。在此，我们报道了从EEBL中获得的六种生物活性化合物具有心脏保护作用；新植酸酶、角鲨烷、植物醇、棕榈酸甲酯、豆甾醇和9，12-十八烷酸甲酯。结论：本研究显示月桂叶提取物作为一种具有足够质量药理学标准的心脏保护剂具有很好的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Medisains

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24 weeks