Piezoelectric micropump with integrated elastomeric check valves: design, performance characterization and primary application for 3D cell culture

IF 3 4区医学 Q3 ENGINEERING, BIOMEDICAL

Biomedical Microdevices Pub Date : 2023-01-17 DOI:10.1007/s10544-022-00645-9

Joseph Benjamin Holman, Xiaolu Zhu, Hao Cheng

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引用次数: 2

Abstract

This paper reports on the study of a piezoelectric actuated micropump with integrated elastomeric check valves that can transport small amounts of fluid in a highly controllable manner. The proposed micropump consists of a piezoelectric actuated fluid chamber with two integrated elastomeric check valves for regulating input and output flow direction, while restricting backflows. The actuation, fluid dynamic response and fluid–structure interactions at various working cycles are studied through a fully coupled multiphysics simulation (solid mechanics, electrostatic and fluid flow). The pump bodies are manufactured by micromachining of PMMA sheets, while the middle elastomeric membrane and diaphragm are fabricated by spin-coating PDMS. The experimental results confirm that the micropump can provide sufficiently low-velocity outflow for biomedical applications between 3.4 – 41.8 µl/min. The performance of the micropump is improved significantly through a convenient geometric modification of an off-the-shelf piezoelectric brass disc. Furthermore, the combination of this micropump with the 3D cell-culture microfluidic chip realizes the dynamic culture of cells encapsulated in 3D hydrogels with a continuous flowing medium, which offers the potential for changing the traditional mode of 3D cell culture with a static supply of nutrition and factors.

Graphical abstract

Abstract Image

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集成弹性止回阀的压电微泵:设计，性能表征和三维细胞培养的主要应用

本文报道了一种集成弹性止回阀的压电驱动微泵的研究，该微泵可以以高度可控的方式输送少量流体。该微泵由一个压电驱动流体腔和两个集成弹性止回阀组成，用于调节输入和输出流向，同时限制回流。通过完全耦合的多物理场模拟(固体力学、静电和流体流动)，研究了不同工作循环下的驱动、流体动力响应和流固耦合作用。泵体采用聚甲基丙烯酸甲酯(PMMA)板材微加工制造，中间弹性体膜和隔膜采用旋涂PDMS制造。实验结果证实，微泵可以在3.4 - 41.8 μ l/min之间为生物医学应用提供足够的低速流出。通过对现成的压电黄铜圆盘进行方便的几何修饰，大大提高了微泵的性能。此外，该微泵与三维细胞培养微流控芯片的结合实现了三维水凝胶中细胞在连续流动介质中的动态培养，为改变传统的静态提供营养和因子的三维细胞培养模式提供了可能。图形抽象

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来源期刊

Biomedical Microdevices 工程技术-工程：生物医学

CiteScore

6.90

自引率

3.60%

发文量

审稿时长

6 months

期刊介绍： Biomedical Microdevices: BioMEMS and Biomedical Nanotechnology is an interdisciplinary periodical devoted to all aspects of research in the medical diagnostic and therapeutic applications of Micro-Electro-Mechanical Systems (BioMEMS) and nanotechnology for medicine and biology. General subjects of interest include the design, characterization, testing, modeling and clinical validation of microfabricated systems, and their integration on-chip and in larger functional units. The specific interests of the Journal include systems for neural stimulation and recording, bioseparation technologies such as nanofilters and electrophoretic equipment, miniaturized analytic and DNA identification systems, biosensors, and micro/nanotechnologies for cell and tissue research, tissue engineering, cell transplantation, and the controlled release of drugs and biological molecules. Contributions reporting on fundamental and applied investigations of the material science, biochemistry, and physics of biomedical microdevices and nanotechnology are encouraged. A non-exhaustive list of fields of interest includes: nanoparticle synthesis, characterization, and validation of therapeutic or imaging efficacy in animal models; biocompatibility; biochemical modification of microfabricated devices, with reference to non-specific protein adsorption, and the active immobilization and patterning of proteins on micro/nanofabricated surfaces; the dynamics of fluids in micro-and-nano-fabricated channels; the electromechanical and structural response of micro/nanofabricated systems; the interactions of microdevices with cells and tissues, including biocompatibility and biodegradation studies; variations in the characteristics of the systems as a function of the micro/nanofabrication parameters.