Localization of symbiotic bacteria in embryogenesis of the lesser grain borer Rhyzopertha dominica and the African powderpost beetle Lyctus africanus (Coleoptera: Bostrichidae)

IF 1.3 4区 农林科学 Q2 ENTOMOLOGY
Bin Hirota, Ryuichi Koga, Takema Fukatsu
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Abstract

Embryogenesis of two pest beetles of the family Bostrichidae, the lesser grain borer Rhyzopertha dominica (Fabricius) and the African powderpost beetle Lyctus africanus Lesne, was investigated, in which the developmental process of the bacteriome formation was particularly focused on using whole-mount fluorescence in situ hybridization targeting 16S rRNA of the bacterial symbiont ‘Candidatus Shikimatogenerans bostrichidophilus’. In R. dominica, we described the following embryonic developmental stages: the superficial cleavage stage, with symbiont cells scattered in the surface region; the germband formation stage, with symbiont cells migrating into the yolk and gathering around the germband; the germband extension stage, with symbiont cells remaining around the germband; the germband retraction stage, with symbiont cells infecting the germband; and the bacteriome formation stage, with paired bacteriomes formed in parallel with dorsal closure. In L. africanus, similar stages of the embryonic development were identified. These results lay the foundation for future studies on the developmental and evolutionary biology of the microbial symbiosis in the Bostrichidae.

Abstract Image

共生细菌在小玉米螟Rhyzopertha dominica和非洲粉蚧Lyctus africanus胚胎发生中的定位(鞘翅目:Bostrichidae)
本文研究了两种bostrichididae科害虫甲虫——小纹螟Rhyzopertha dominica (Fabricius)和非洲粉虫Lyctus africanus Lesne的胚胎发生过程,重点研究了细菌共生菌Candidatus Shikimatogenerans bostrichidophilus的16S rRNA全载荧光原位杂交技术。在多米尼加,我们描述了以下胚胎发育阶段:表面分裂阶段,共生体细胞分散在表面区域;胚带形成阶段,共生细胞向卵黄内迁移,聚集在胚带周围;种带延伸期,共生细胞留在种带周围;种带收缩期,共生细胞侵染种带;而细菌组形成阶段,以成对的细菌组平行形成,背向闭合。在L. africanus中,发现了类似的胚胎发育阶段。这些研究结果为今后对虫科微生物共生的发育和进化生物学研究奠定了基础。
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来源期刊
CiteScore
2.70
自引率
7.70%
发文量
37
审稿时长
6-12 weeks
期刊介绍: Applied Entomology and Zoology publishes articles concerned with applied entomology, applied zoology, agricultural chemicals and pest control in English. Contributions of a basic and fundamental nature may be accepted at the discretion of the Editor. Manuscripts of original research papers, technical notes and reviews are accepted for consideration. No manuscript that has been published elsewhere will be accepted for publication.
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