Varroa destructor (Acari: Varroidea) populations from Southern Iran belong to haplotype K of the mitochondrial COI

IF 0.7 Q4 ENTOMOLOGY
Zahra Hajializadeh, M. Asadi, K. Ahmadi, A. Balvasi
{"title":"Varroa destructor (Acari: Varroidea) populations from Southern Iran belong to haplotype K of the mitochondrial COI","authors":"Zahra Hajializadeh, M. Asadi, K. Ahmadi, A. Balvasi","doi":"10.22073/PJA.V8I2.43363","DOIUrl":null,"url":null,"abstract":"A molecular characterization based on mitochondrial cytochrome oxidase subunit I gene was used to genotype central-southern Iranian populations of Varroa destructor for the first time. A 570 bp segement was amplified by Polymerase Chain Reaction and digested with two restriction enzymes. Digestion of PCR products with XhoI produced fragments of 300 and 270 bp in all samples, whereas digestion with SacI did not produce any digested fragments. Results suggest that all samples belong to the K haplotype previously described for V. destructor in the literature. A total of 22 sequences of Iranian samples (418bp) were registered for the first time in NCBI.","PeriodicalId":37567,"journal":{"name":"Persian Journal of Acarology","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.22073/PJA.V8I2.43363","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Persian Journal of Acarology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22073/PJA.V8I2.43363","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

A molecular characterization based on mitochondrial cytochrome oxidase subunit I gene was used to genotype central-southern Iranian populations of Varroa destructor for the first time. A 570 bp segement was amplified by Polymerase Chain Reaction and digested with two restriction enzymes. Digestion of PCR products with XhoI produced fragments of 300 and 270 bp in all samples, whereas digestion with SacI did not produce any digested fragments. Results suggest that all samples belong to the K haplotype previously described for V. destructor in the literature. A total of 22 sequences of Iranian samples (418bp) were registered for the first time in NCBI.
来自伊朗南部的破坏瓦螨(蜱螨亚纲:瓦螨总科)种群属于线粒体COI单倍型K
利用线粒体细胞色素氧化酶亚基I基因的分子特征,首次对伊朗中南部种群进行了基因分型。用聚合酶链反应扩增出570 bp的片段,并用两种限制性内切酶进行酶切。用XhoI酶切的PCR产物在所有样品中都产生了300和270 bp的片段,而用SacI酶切的PCR产物没有产生任何酶切片段。结果表明,所有样本均属于先前文献中描述的破坏性v的K单倍型。在NCBI中首次登记到22个伊朗样本序列(418bp)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Persian Journal of Acarology
Persian Journal of Acarology Agricultural and Biological Sciences-Insect Science
CiteScore
1.60
自引率
30.80%
发文量
0
审稿时长
20 weeks
期刊介绍: Persian Journal of Acarology (PJA) is a peer-reviewed international journal of the Acarological Society of Iran for publication of high quality papers on any aspect of Acarology including mite and tick behavior, biochemistry, biology, control, ecology, evolution, morphology, physiology, systematics and taxonomy. All manuscripts will be subjected to peer review before acceptance.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信