Nephroprotective effect of Berberis baluchistanica against gentamicin-induced nephrotoxicity in rabbit

IF 0.9 4区医学 Q4 PHARMACOLOGY & PHARMACY

Bangladesh Journal of Pharmacology Pub Date : 2018-07-21 DOI:10.3329/BJP.V13I3.36621

Samreen Pervez, M. Saeed, H. Khan, M. Shahid, I. Ullah

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引用次数: 8

Abstract

The present study was conducted to explore the protective effect of B. baluchistanica against gentamicin-induced renal toxicity in rabbits. Phytochemical investigations lead to the isolation of berberine and palmatine. The crude hydro-methanolic extract at various doses (100, 200 and 300 mg/kg body weight) elicited strong nephroprotective effects by restoring various biomarkers which were deranged by gentamicin such as creatinine, urea, serum uric acid levels (p<0.001) in plasma and urine output creatinine clearance, urinary protein and γ-glutamyl transferase level (p<0.001) in urine in a dose dependent manner. The mediators involved in oxidative stress such malondialdehyde, reduced glutathione, glutathione peroxidase, and catalase levels were significantly (P˂0.05-0.001) modulated in kidney tissue homogenate. Correspondingly, there is a significant (P<0.05) recovery in kidney weight and % loss in body weight compare to GM group. From these results, it is possible that B. baluchistanica exhibited protective effect mediated through the presence of berberine and palmatine.Video Clip of Methodology:Histopathology: 7 min 24 sec Full Screen Alternate

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俾路支小檗对庆大霉素肾毒性的保护作用

本研究旨在探讨巴鲁司坦卡对庆大霉素肾毒性的保护作用。植物化学研究导致了黄连素和巴马汀的分离。不同剂量（100、200和300mg/kg体重）的粗氢甲醇提取物通过恢复被庆大霉素破坏的各种生物标志物，如肌酸酐、尿素、血浆中的血清尿酸水平（p<0.001）和尿肌酐清除率，尿蛋白和γ-谷氨酰转移酶水平呈剂量依赖性（p＜0.001）。参与氧化应激的介质如丙二醛、还原型谷胱甘肽、谷胱甘肽过氧化物酶和过氧化氢酶水平在肾组织匀浆中受到显著调节（P＜0.05～0.01）。相应地，与GM组相比，肾脏重量有显著的恢复（P<0.05），体重减轻了%。从这些结果来看，巴鲁司坦卡杆菌可能表现出通过黄连素和巴马汀的存在介导的保护作用。方法学视频剪辑：组织病理学：7分24秒全屏交替

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来源期刊

Bangladesh Journal of Pharmacology PHARMACOLOGY & PHARMACY-

CiteScore

1.50

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is an open access, video component, peer-reviewed journal of the Bangladesh Pharmacological Society (BDPS). A scholarly publication is defined ''open access'' when there are no financial, legal or technical barriers to accessing it. Anyone can read, download, copy, distribute, print or use it in educational purpose within the legal agreements. Peer review of a manuscript means the evaluation of research work by one or more people with similar competences. It can be open or blind. We do the single-blind peer review. Our readers want to know the methodology used by the researchers which is very important to get the results. The video clip of one of the methodologies is prepared by the authors and submit it to publish.