Susceptibilidad del PTZ en ratones viejos y jóvenes. Diferencias entre un protocolo kindling convulsivo y subconvulsivo

Abstract

Introduction

Kindling Model consist sequential injections of a dose Pentylenetetrazole causing spontaneous seizures. (ES)

Objectives

Compare convulsive and subconvulsive effect in old and young mice, to evaluate the differences between ichthyogenesis and epileptogenesis.

Methods

Rapid kindling model was performed using pentylenetetrazole in C57BL/6 mice. Using convulsive doses (C) (75 mg/kg in one injection) and sub-convulsive (SC) (10 mg/kg every 5 minutes for 30 minutes, for a total of 5 injections). Four groups were formed, young mouse (<32 weeks; C = 8; SC = 9) and 16 Old mouse (>32 weeks; C = 8; SC = 8). Using Racine Score. Descriptive statistics.

Results

Has been made 16 C protocols and 17 SC protocols. The latency time to phase 1 in sec. it is lower in old C (50.5 sec) than in young C (170.3). In SC, it was higher in the old group 170.3 vs young 105.5 (P = 0.001). Young C group presented longer time during Phase 1 to 3, and old C group during phase 4 and 5. Young SC presented longer duration in phase 2, unlike old SC with linear duration in different SR phases (P = 0.001). There were no differences in spontaneous death in all groups.

Conclusion

Latency time to Phase 1 was shorter in old convulsives and longer than young SC, indicative greater ichthyogenesis and less epileptogenesis in the old population. Tendency in the elderly to stay longer in phases 4 and 5 in convulsive doses suggests a greater tendency to ichthyogenesis and greater severity of spontaneous seizures.