Pumpkin seed ethanolic extract protects against escitalopram-induced reproductive toxicity in male mice

IF 0.5 Q4 REPRODUCTIVE BIOLOGY
Agrawal Karuna, Shahani Lata, B. Pradeep
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Abstract

Objective: To investigate the protective role of pumpkin seed ethanolic extract against escitalopram-induced reproductive toxicity in male mice. Methods: Swiss albino male mice were randomly divided into five groups with six mice in each group. Group I received normal water orally, Group II, III, IV and V received escitalopram oxalate (10 mg/kg), pumpkin seed extract (300 mg/kg) plus escitalopram oxalate (10 mg/kg), escitalopram oxalate (20 mg/kg), and pumpkin seed extract (300 mg/kg) plus escitalopram oxalate (20 mg/kg), respectively. All test doses were continuously administered orally once daily per animal body weight for 30 days and 60 days. Body weight and sexual organ weight were evaluated on day 31 and 61. Effects of pumpkin seed extract on sperm parameters, biochemical parameters and histology of testis were also investigated. Results: Escitalopram 10 or 20 mg/kg caused reproductive toxicity in male mice after 30 and 60 days of treatment. However, simultaneous administration of escitalopram oxalate (10 or 20 mg/kg) with pumpkin seed extract (300 mg/kg) attenuated escitalopram-induced testicular toxicity. Significant increase in the body weight and relative organ weight was observed. Sperm count, sperm motility and viability significantly increased (P<0.05). The histopathological alterations caused by escitalopram was also ameliorated. Conclusions: Ethanolic extract of pumpkin seeds (300 mg/kg body weight) protects again reproductive toxicity induced by escitalopram. Therefore, dietary intake of pumpkin seed extract might be useful for male patients who expose to antidepressant drug due to depression.
南瓜籽乙醇提取物对艾司西酞普兰诱导的雄性小鼠生殖毒性的保护作用
目的:研究南瓜子乙醇提取物对艾司西酞普兰雄性小鼠生殖毒性的保护作用。方法:将瑞士白化病雄性小鼠随机分为5组,每组6只。第一组口服正常水,第二组、第三组、第四组和第五组分别接受草酸艾西酞普兰(10 mg/kg)、南瓜籽提取物(300 mg/kg)加草酸艾西他普兰(10 mg/kg)、草酸艾西特普兰(20 mg/kg)和南瓜籽提取物。所有试验剂量连续口服,每只动物体重每天一次,持续30天和60天。在第31天和第61天评估体重和性器官重量。研究了南瓜籽提取物对精子参数、睾丸生化参数和组织学的影响。结果:依西酞普兰10或20mg/kg给药30和60天后对雄性小鼠产生生殖毒性。然而,草酸艾司西酞普兰(10或20 mg/kg)与南瓜籽提取物(300 mg/kg)同时给药可减轻艾司西普兰诱导的睾丸毒性。观察到体重和相对器官重量显著增加。精子计数、精子活力和活力显著增加(P<0.05),艾司西酞普兰引起的组织病理学改变也得到改善。结论:南瓜籽乙醇提取物(300mg/kg体重)可再次保护艾司西酞普兰引起的生殖毒性。因此,从饮食中摄入南瓜籽提取物可能对因抑郁症而接触抗抑郁药物的男性患者有用。
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来源期刊
Asian Pacific Journal of Reproduction
Asian Pacific Journal of Reproduction Veterinary-Veterinary (all)
CiteScore
1.70
自引率
0.00%
发文量
588
审稿时长
9 weeks
期刊介绍: The journal will cover technical and clinical studies related to health, ethical and social issues in field of Gynecology and Obstetrics. Articles with clinical interest and implications will be given preference.
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