Persistence of MRSA and ESBL-producing E. coli and K. oxytoca in river water

Sarah M. Essert , Nicole Zacharias , Tabea Precht , Debbie Pankratz , Karolin Funken , Nico T. Mutters , Thomas Kistemann , Christiane Schreiber
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Abstract

Due to climate change precipitation patterns are changing in terms of frequency and quantity in Germany. As a result, prolonged dry weather can lead to extremely low flow rates in river systems with large portions of treated wastewater. However, storm events and heavy rainfall events are increasing too, which may lead to higher frequencies of combined sewer overflows. Increased microbial pollution (e.g. coliform bacteria, clostridia, faecal streptococci) of waterbodies after heavy precipitation events was demonstrated in several studies. Pathogenic multi-resistant bacteria enter waterbodies via sewer systems. The persistence potential of such pathogens in aquatic environments is mainly still unknown. This study investigated the growth behaviour of Gram-negative extended spectrum beta-Lactamase-producing Klebsiella oxytoca and Escherichia coli and Gram-positive methicillin-resistant Staphylococcus aureus in river water. The concentrations of these antibiotic-resistant bacteria were monitored in batch experiments using river water differently impacted by wastewater. Staphylococcus aureus persisted for at least 3 days at incubation temperatures of 8 and 18 °C. The persistence of Koxytoca and Ecoli was shown for at least 37 days at both temperatures. In the river water at 8 °C, bacteria concentrations decreased slower, suggesting diminished persistence of ARB (antibiotic-resistant bacteria) with increasing temperatures. Initial concentrations of ARB and other pathogens in river water corresponded to the impact of wastewater. However, significant effects on the persistence itself by wastewater impact could not be shown for the bacteria species tested. Further studies should pursue our approaches concerning the persistence of pathogenic ARB in freshwater to assess the health risk for humans and animals.

Abstract Image

耐甲氧西林金黄色葡萄球菌和产esbl的大肠杆菌和脱氧梭菌在河水中的持久性
由于气候变化,降水模式在德国的频率和数量方面正在发生变化。因此,长时间的干燥天气可能导致河流系统的流量极低,其中含有大量处理过的废水。然而,暴雨事件和强降雨事件也在增加,这可能导致合流下水道溢流的频率更高。几项研究表明,强降水事件后水体的微生物污染(如大肠菌群、梭状芽孢杆菌、粪便链球菌)增加。致病性多重耐药细菌通过下水道系统进入水体。这些病原体在水生环境中的持久性潜力主要仍然未知。本研究研究了革兰氏阴性产广谱β -内酰胺酶克雷伯菌和大肠杆菌以及革兰氏阳性耐甲氧西林金黄色葡萄球菌在河水中的生长行为。利用受不同污水影响程度的河水进行了批量试验,监测了这些耐药菌的浓度。金黄色葡萄球菌在8°C和18°C的孵育温度下可存活至少3天。结果表明,在这两种温度下,后芽胞杆菌和大肠杆菌的持久性至少为37天。在8°C的河水中,细菌浓度下降较慢,表明ARB(抗生素耐药细菌)的持久性随着温度的升高而降低。河水中ARB和其他病原体的初始浓度与废水的影响相对应。然而,污水影响对所测细菌种类的持久性本身没有显着影响。进一步的研究应遵循我们关于淡水中致病性ARB持续存在的方法,以评估对人类和动物的健康风险。
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来源期刊
Hygiene and environmental health advances
Hygiene and environmental health advances Environmental Science (General)
CiteScore
1.10
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0.00%
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0
审稿时长
38 days
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