Effects of Garlicin on Proliferation and Differentiation of HL-60 Cells

IF 0.4 4区医学 Q4 NUTRITION & DIETETICS

Current Topics in Nutraceutical Research Pub Date : 2023-04-07 DOI:10.37290/ctnr2641-452x.21:109-116

Jinshen Wang, Chun-qing Bu, Yanyan Wang, Haiyan Ye, Yue Han, Daogang Qin

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引用次数: 0

Abstract

We have explored the effect of garlicin, a biologically active compound isolated from garlic, on the proliferation and differentiation of HL-60 leukemia cells. To this end, we measured the effect of garlicin on cell proliferation, cell morphology, cell cycle, and the expression cluster of differentiation molecules 11b and 15, B-cell lymphoma 2 protein, and BCL2-associated X proteins. All-trans retinoic acid and arsenic trioxide treatments served as positive controls for comparison. Results show that garlicin significantly inhibited the proliferation of HL-60 cells and blocked most cells in the G0/G1 phase, with an effect stronger than all-trans retinoic acid or arsenic trioxide treatments. The ability to induce cell maturation and differentiation was like that of all-trans retinoic acid, which may play a role by downregulating the expression of B-cell lymphoma 2 protein and upregulating the expression of BCL2-associated X protein. In conclusion, garlicin significantly inhibits the proliferation and induces differentiation of HL-60 cells, and thus it may become a new clinically viable differentiation-inducing agent.

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大蒜素对HL-60细胞增殖和分化的影响

我们研究了大蒜素对HL-60白血病细胞增殖和分化的影响。为此，我们测量了大蒜素对细胞增殖、细胞形态、细胞周期以及分化分子11b和15、b细胞淋巴瘤2蛋白和bcl2相关X蛋白表达簇的影响。全反式维甲酸和三氧化二砷处理作为阳性对照进行比较。结果表明，大蒜素显著抑制HL-60细胞的增殖，并在G0/G1期阻断大部分细胞，其作用强于全反式维甲酸和三氧化二砷处理。其诱导细胞成熟和分化的能力与全反式维甲酸相似，可能通过下调b细胞淋巴瘤2蛋白的表达和上调bcl2相关X蛋白的表达来发挥作用。综上所述，大蒜素能显著抑制HL-60细胞的增殖并诱导其分化，可能成为一种新的临床可行的分化诱导剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Topics in Nutraceutical Research 医学-药学

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Current Topics in Nutraceutical Research is an international, interdisciplinary broad-based peer reviewed scientific journal for critical evaluation of research on chemistry, biology and therapeutic applications of nutraceuticals and functional foods. The major goal of this journal is to provide peer reviewed unbiased scientific data to the decision makers in the nutraceutical and food industry to help make informed choices about development of new products. To this end, the journal will publish two types of review articles. First, a review of preclinical research data coming largely from animal, cell culture and other experimental models. Such data will provide basis for future product development and/or human research initiatives. Second, a critical evaluation of current human experimental data to help market and deliver the product for medically proven use. This journal will also serve as a forum for nutritionists, internists, neurologists, psychiatrists, and all those interested in preventive medicine. The common denominator of all of the topic to be covered by the journal must include nutraceuticals and/functional food. The following is an example of some specific areas that may be of interest to the journal. i) Role of vitamins, minerals, antioxidants and phytonutrients on cardiovascular health, cancer, diabetes, ocular health, mental health, men’s health, women’s health, infant nutrition, ii) Role of herbals on human health, iii) Dietary supplements and sleep, iv) Components of diet that may have beneficial effect on human health, v) regulation of apoptosis and cell viability, vi) Isolation and characterization of bioactive components from functional foods, vii) Nutritional genomics, and viii) Nutritional proteomics.