Using non-destructive sampling to evaluate the population genomic status of captive Brown Eared Pheasants

IF 1.6 2区 生物学 Q1 ORNITHOLOGY
Pengcheng Wang , Ping Hu , Jinping Zhang , Lixia Zhang , Jing Zhang , Zhengwang Zhang
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引用次数: 0

Abstract

Evaluating the genetic status of threatened species is an essential task in conservation genetics. However, the genetic status of threatened species has been mostly evaluated through techniques that fail to estimate genetic diversity at the whole genomic level. Next generation sequencing can meet this demand, but high quality samples such as blood or muscle tissues are required. However, it is difficult to collect such samples from threatened species because sampling work may impact their health. Therefore, it is essential to design a workflow to evaluate the whole genomic status of threatened species using non-destructive sampling. Even though non-destructive sampling has been used in traditional barcoding technique, the barcoding technique cannot evaluate the whole genomic status. Brown Eared Pheasant (Crossoptilon mantchuricum) is an endangered species, with captive populations maintained in Taiyuan Zoo, China, and Europe. However, the genetic diversity, inbreeding pattern, and mutation load of these two populations are unclear. To uncover the genetic status of these two captive populations, we applied 2b-RAD technology to evaluate the genomic status of these populations using feathers as samples. The feathers could be collected by non-destructive sampling. The results indicate that the Taiyuan Zoo population has a lower genetic diversity and higher inbreeding coefficient than the European population. The Taiyuan Zoo population has lethal mutations when homozygous. The current project uses a non-destructive sampling technique to evaluate the whole genomic status of the two captive populations, providing a paradigm for conservation genetics, which will facilitate the development of conservation biology.

采用非破坏性抽样方法评价圈养褐耳雉种群基因组状况
评估濒危物种的遗传状况是保护遗传学的一项重要工作。然而,对濒危物种遗传状况的评估大多是通过无法在全基因组水平上评估遗传多样性的技术进行的。下一代测序可以满足这一需求,但需要高质量的血液或肌肉组织样本。然而,很难从受威胁物种中收集这种样本,因为采样工作可能影响它们的健康。因此,有必要设计一个工作流程来评估受威胁物种的全基因组状态,使用非破坏性采样。尽管传统的条形码技术采用非破坏性采样,但条形码技术无法评估整个基因组的状态。棕耳雉(Crossoptilon mantchuricum)是一种濒危物种,在中国太原动物园和欧洲都有圈养种群。然而,这两个种群的遗传多样性、近交模式和突变负荷尚不清楚。为了揭示这两个圈养种群的遗传状况,我们以羽毛为样本,应用2b-RAD技术对这两个种群的基因组状况进行了评估。羽毛可以通过非破坏性取样收集。结果表明,与欧洲种群相比,太原动物园种群的遗传多样性较低,近交系数较高。太原动物园种群纯合时发生致死性突变。目前的项目采用非破坏性采样技术来评估两个圈养种群的整体基因组状况,为保护遗传学提供了一个范例,这将促进保护生物学的发展。
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来源期刊
Avian Research
Avian Research ORNITHOLOGY-
CiteScore
2.90
自引率
16.70%
发文量
456
审稿时长
46 days
期刊介绍: Avian Research is an open access, peer-reviewed journal publishing high quality research and review articles on all aspects of ornithology from all over the world. It aims to report the latest and most significant progress in ornithology and to encourage exchange of ideas among international ornithologists. As an open access journal, Avian Research provides a unique opportunity to publish high quality contents that will be internationally accessible to any reader at no cost.
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