The Toxic Influence of Excess Free Iron on Red Blood Cells in the Biophysical Experiment: An In Vitro Study

IF 3.4 Q2 TOXICOLOGY

Journal of Toxicology Pub Date : 2022-02-26 DOI:10.1155/2022/7113958

E. Kozlova, E. Sherstyukova, V. Sergunova, A. Kozlov, O. Gudkova, V. Inozemtsev, A. Chernysh

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引用次数: 8

Abstract

Iron is needed for life-essential processes, but free iron overload causes dangerous clinical consequences. The study of the role of red blood cells (RBCs) in the influence of excess free iron in the blood on the pathological consequences in an organism is relevant. Here, in a direct biophysical experiment in vitro, we studied the action of free iron overload on the packed red blood cell (pRBC) characteristics. In experiments, we incubated pRBCs with the ferrous sulfate solution (Fe2+). Wе used free iron in a wide range of concentrations. High Fe2+ concentrations made us possible to establish the pattern of the toxic effect of excess iron on pRBCs during a reduced incubation time in a biophysical experiment in vitro. It was found that excess free iron causes changes in pRBC morphology, the appearance of bridges between cells, and the formation of clots, increasing the membrane stiffness and methemoglobin concentration. We created a kinetic model of changes in the hemoglobin derivatives. The complex of simultaneous distortions of pRBCs established in our experiments can be taken into account when studying the mechanism of the toxic influence of excess free iron in the blood on pathological changes in an organism.

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生物物理实验中过量游离铁对红细胞的毒性影响:一项体外研究

铁是生命过程所必需的，但游离铁超载会导致危险的临床后果。研究红细胞(rbc)在血液中过量游离铁对机体病理后果的影响中的作用是相关的。本文通过体外直接生物物理实验，研究了游离铁超载对填充红细胞(pRBC)特性的影响。在实验中，我们用硫酸亚铁溶液(Fe2+)培养红细胞。我们使用不同浓度的游离铁。在体外生物物理实验中，高Fe2+浓度使我们能够在较短的孵育时间内建立过量铁对红细胞的毒性作用模式。研究发现，过量的游离铁会导致pRBC形态的改变、细胞间桥的出现和血栓的形成，增加膜刚度和高铁血红蛋白浓度。我们建立了血红蛋白衍生物变化的动力学模型。在研究血液中过量游离铁对生物体病理变化的毒性影响机制时，可以考虑在我们的实验中建立的红细胞同时扭曲的复杂性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Toxicology TOXICOLOGY-

CiteScore

5.50

自引率

3.40%

发文量

审稿时长

10 weeks

期刊介绍： Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences. The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological medicine, risk assessment, safety evaluation, and environmental health.