Identification of the Side Population Associated with ATP-Binding Cassette Transporters Activity Using Imaging Flow Cytometry

IF 0.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Pub Date : 2021-08-16 DOI:10.1134/S1990750821030033

A. M. Gisina, Y. S. Kim, K. N. Yarygin, A. Yu. Lupatov

引用次数: 1

Abstract—

DyeCycle Violet efflux, caused by ATP-binding cassette transporters activity, was analyzed in human colorectal adenocarcinoma cell lines SW480, HT-29, Caco-2 by means of a FACSAria III flow cytometer and an ImageStreamX Mk II imaging flow cytometer. Besides similarity of cytometry data obtained by two instruments, the use of imaging flow cytometry made it possible to characterize the morphology of side population cells, as well as morphology of other cell populations differing in the degree of dye accumulation. The population of cells, which are smaller than the side population cells and practically do not take the dye, is of the special interest. Probably, this population may contribute to the tumor resistance to chemotherapy.

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利用成像流式细胞术鉴定与atp结合盒转运体活性相关的侧群

利用FACSAria III型流式细胞仪和ImageStreamX Mk II型成像流式细胞仪，分析了atp结合盒转运体活性引起的dyecycle紫罗兰外排在人结直肠癌细胞系SW480、HT-29、Caco-2中。除了两种仪器获得的细胞术数据相似外，使用成像流式细胞术可以表征侧群体细胞的形态，以及染料积累程度不同的其他细胞群体的形态。细胞群比侧细胞群小，实际上不染染料，这是特别有趣的。可能，这一群体可能有助于肿瘤对化疗的抵抗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry covers all major aspects of biomedical chemistry and related areas, including proteomics and molecular biology of (patho)physiological processes, biochemistry, neurochemistry, immunochemistry and clinical chemistry, bioinformatics, gene therapy, drug design and delivery, biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine. The journal also publishes review articles. All issues of the journal usually contain solicited reviews.