Evaluation of total sialic acid, paraoxonase activity and malondialdehyde in cows with subclinical paratuberculosis

IF 0.4 4区农林科学 Q4 VETERINARY SCIENCES

Journal of the Hellenic Veterinary Medical Society Pub Date : 2022-12-15 DOI:10.12681/jhvms.25689

E. Akyuz, A. Kükürt, M. Makav, E. Gökçe, H. Eroğlu, H. Deveci, G. Gökce, M. Karapehlivan

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引用次数: 1

Abstract

In this study, it was aimed to evaluate total sialic acid (TSA), paraoxonase activity (PON1), malondialdehyde (MDA) and some serum biochemical parameters in cows with subclinical paratuberculosis. Paratuberculosis (PT) test was performed on 500 cows aged 2-6 years by ELISA method. Twenty-four cows diagnosed with paratuberculosis constituted the PT group and 12 healthy cows constituted the control group. According to the results, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and UREA levels were found to be statistically higher (p <0.05) in the group with paratuberculosis compared to the control group. Iron (Fe) levels were found to be lower than the control group (p> 0.05). Compared to the control group, there was a decrease in PON1 activity and a significant increase in TSA and MDA levels in the PT group (p <0.05). In conclusion, in subclinical paratuberculosis infection, the long incubation period and the absence of clinical findings until advanced stages constitute a handicap. However, our findings show that the determination of lipid peroxidation (MDA), PON1 activity and TSA concentration caused by the inflammatory and oxidative stress state caused by the infection can be used as new biomarkers for this infection.

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亚临床副结核奶牛总唾液酸、对氧磷酶活性和丙二醛的评价

本研究旨在评估亚临床副结核奶牛的总唾液酸（TSA）、对氧磷酶活性（PON1）、丙二醛（MDA）和一些血清生化参数。采用ELISA法对500头2-6岁奶牛进行了肺结核（PT）试验。24头诊断为副结核的奶牛组成PT组，12头健康奶牛组成对照组。结果显示，丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶和UREA水平均高于对照组（p＜0.05）。与对照组相比，PT组PON1活性下降，TSA和MDA水平显著升高（p＜0.01）。总之，在亚临床副结核感染中，潜伏期长以及直到晚期才有临床发现是一个障碍。然而，我们的研究结果表明，由感染引起的炎症和氧化应激状态引起的脂质过氧化（MDA）、PON1活性和TSA浓度的测定可以作为这种感染的新生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Hellenic Veterinary Medical Society VETERINARY SCIENCES-

CiteScore

0.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of the Hellenic Veterinary Medical Society (J Hellenic Vet Med Soc) is a quarterly peer-reviewed journal that publishes articles in all aspects of veterinary science and related disciplines. It is published by the Hellenic Veterinary Medical Society and is indexed in the Web of Science and in Scopus. There are no publication fees in the journal. Authors considering submitting manuscripts for evaluation and publication are requested to read carefully the instructions for authors and fully comply with them. Non-complying manuscripts may be returned to the corresponding author for formatting.