Derivation and Differentiation of Human Pluripotent Stem Cells in Microfluidic Devices.

IF 12.8 1区工程技术 Q1 ENGINEERING, BIOMEDICAL

Annual Review of Biomedical Engineering Pub Date : 2022-04-04 DOI:10.1146/annurev-bioeng-092021-042744

C. Luni, O. Gagliano, N. Elvassore

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引用次数: 8

Abstract

An integrative approach based on microfluidic design and stem cell biology enables capture of the spatial-temporal environmental evolution underpinning epigenetic remodeling and the morphogenetic process. We examine the body of literature that encompasses microfluidic applications where human induced pluripotent stem cells are derived starting from human somatic cells and where human pluripotent stem cells are differentiated into different cell types. We focus on recent studies where the intrinsic features of microfluidics have been exploited to control the reprogramming and differentiation trajectory at the microscale, including the capability of manipulating the fluid velocity field, mass transport regime, and controllable composition within micro- to nanoliter volumes in space and time. We also discuss studies of emerging microfluidic technologies and applications. Finally, we critically discuss perspectives and challenges in the field and how these could be instrumental for bringing about significant biological advances in the field of stem cell engineering. Expected final online publication date for the Annual Review of Biomedical Engineering, Volume 24 is June 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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人多能干细胞在微流控装置中的衍生与分化。

基于微流体设计和干细胞生物学的综合方法能够捕捉支持表观遗传学重塑和形态发生过程的时空环境进化。我们研究了包括微流体应用的大量文献，其中人类诱导的多能干细胞从人类体细胞开始衍生，并且人类多能干细胞分化为不同的细胞类型。我们专注于最近的研究，在这些研究中，微流体的内在特征已被用来在微观尺度上控制重编程和分化轨迹，包括在空间和时间上操纵流体速度场、质量传输机制和微升至纳升体积内的可控组分的能力。我们还讨论了新兴微流体技术和应用的研究。最后，我们批判性地讨论了该领域的观点和挑战，以及这些观点和挑战如何有助于在干细胞工程领域取得重大生物学进展。《生物医学工程年度评论》第24卷预计最终在线出版日期为2022年6月。请参阅http://www.annualreviews.org/page/journal/pubdates用于修订估算。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annual Review of Biomedical Engineering 工程技术-工程：生物医学

CiteScore

18.80

自引率

0.00%

发文量

期刊介绍： Since 1999, the Annual Review of Biomedical Engineering has been capturing major advancements in the expansive realm of biomedical engineering. Encompassing biomechanics, biomaterials, computational genomics and proteomics, tissue engineering, biomonitoring, healthcare engineering, drug delivery, bioelectrical engineering, biochemical engineering, and biomedical imaging, the journal remains a vital resource. The current volume has transitioned from gated to open access through Annual Reviews' Subscribe to Open program, with all articles published under a CC BY license.