Determination of optimal plant growth regulators for breaking seed dormancy and micropropagation of Sorbus aucuparia L.

IF 0.7 4区农林科学 Q3 FORESTRY

Baltic Forestry Pub Date : 2023-04-03 DOI:10.46490/bf679

D. Dinçer

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引用次数: 0

Abstract

Sorbus aucuparia L. is an important forest tree used in the reforestation of high-altitude mountainous areas, which carries significant pharmaceutical, industrial, ornamental, food, and ecological properties. However, the seed propagation and micropropagation of mature trees of S. aucuparia L., presents various difficulties which are integral aspects for the re-propagation and breeding of novel cultivars. In this study, we isolated the mature embryo-containing seeds of selected varieties of S. aucuparia L. from their seed coat and used them directly as explants in vitro to investigate and determine the optimal dose of cytokinin in breaking seed dormancy and micropropagation. 3 mg/L of benzyl adenine (BA) in addition to different concentrations and combinations of kinetin (0.5, 1, and 2 mg/L), indole-3-butyric acid (IBA) (0, 1, 0.5, and 1 mg/L), and 1-naphthalene acetic acid (NAA) (0, 1, 0.5, and 1 mg/L) were applied within a Murashige and Skoog (1962) (MS) medium at germination inhibition, shoot elongation, and shoot proliferation. In the MS mediums containing BA and kinetin, germination was achieved at the end of 1 week, and shoot proliferation was achieved at the end of 3 weeks. The most successful germination (96%), tallest shoot length (mean 5.1 cm), most shoot proliferation (mean 7.2 pieces), and number of nodes (mean 9.7 pieces) were identified in the MS containing 3 mg/L BA and 1 mg/L kinetin. Direct root formation with shoot elongation occurred in 25% of explants which germinated in the MS medium. For shoots propagated without roots in this medium, 62% of these achieved rooting at the highest dose of 1 mg/L indole-3-acetic acid (IAA) using a two-stage rooting method. Rooted shoots were successfully transferred to an ex vitro medium. These results provide a basis for breaking seed dormancy of selected Sorbus L. genotypes quicker, leading to more effective clonal production. Keywords: Sorbus aucuparia L., rowanberry, tissue culture, seed dormancy, micropropagation, in vitro

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花楸种子破休眠和微繁殖最佳植物生长调节剂的确定。

花楸。是一种用于高海拔山区重新造林的重要林木，具有重要的药用、工业、观赏、食品和生态特性。然而，对S。 Aucupatia L。，提出了各种困难，这些困难是新品种再繁殖和育种的组成部分。在本研究中，我们分离出了S。 Aucupatia L。并将其直接用作离体外植体，研究和确定细胞分裂素在打破种子休眠和微繁殖中的最佳剂量。3. mg/L苄基腺嘌呤（BA）以及不同浓度和组合的激动素（0.5、1和2 mg/L）、吲哚-3-丁酸（IBA）（0、1、0.5和1 mg/L）和1-萘乙酸（NAA）（0、1、0.5和1 mg/L）在Murashige和Skoog（1962）（MS）培养基中进行发芽抑制、芽伸长和芽增殖。在含有BA和激动素的MS培养基中，在1周末实现发芽，在3周末实现芽增殖。发芽最成功（96%），芽长最高（平均5.1 cm），大多数芽增殖（平均7.2个）和节数（平均9.7个）在含有3 mg/L BA和1 mg/L激动素。在MS培养基中发芽的外植体中，25%的外植体发生了具有芽伸长的直接生根。对于在该培养基中无根繁殖的芽，62%的芽在最高剂量1 mg/L吲哚-3-乙酸（IAA）。生根的芽被成功地转移到体外培养基中。这些结果为打破花楸种子休眠提供了依据。基因型更快，导致更有效的克隆生产。关键词：花楸。，罗望子，组织培养，种子休眠，微繁殖，体外

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来源期刊

Baltic Forestry 农林科学-林学

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The journal welcomes the original articles as well as short reports, review papers on forestry and forest science throughout the Baltic Sea region and elsewhere in the area of boreal and temperate forests. The Baltic Sea region is rather unique through its intrinsic environment and distinguished geographical and social conditions. A temperate climate, transitional and continental, has influenced formation of the mixed coniferous and deciduous stands of high productivity and biological diversity. The forest science has been affected by the ideas from both the East and West. In 1995, Forest Research Institutes and Universities from Estonia, Latvia and Lithuania joined their efforts to publish BALTIC FORESTRY.