Intracytoplasmic Trafficking of Nanoparticles based on Hyaluronic Acid and Chitosan for Targeted Delivery of Anticancer Drugs

Q3 Biochemistry, Genetics and Molecular Biology

Biointerface Research in Applied Chemistry Pub Date : 2022-09-11 DOI:10.33263/briac134.344

{"title":"Intracytoplasmic Trafficking of Nanoparticles based on Hyaluronic Acid and Chitosan for Targeted Delivery of Anticancer Drugs","authors":"","doi":"10.33263/briac134.344","DOIUrl":null,"url":null,"abstract":"Cancer diseases are characterized by high incidence and mortality worldwide. The main problem in cancer therapy is the lack of specificity of anti-cancer drugs. Therefore, the development of new methods of targeted delivery of anti-cancer drugs is an urgent task in oncology. Nanoparticles from hyaluronic acid and chitosan (HA:CS) were obtained using ionotropic gelation technology. The size of the nanoparticles was investigated using dynamic light scattering. Nanoparticles were obtained of a size of 100-400 nm. A physical association method has been developed for encapsulating nanoparticles with doxorubicin, a well-known antitumor drug, and dinitrosyl iron complex (DNIC; donor of nitric oxide). Using the method of dynamic light scattering, the surface potential of nanoparticles was measured. It was found that the resulting nanoparticles (HA-DOX:CS) were stable and had a surface potential of -45.6 meV. Using the method of confocal and FLIM microscopy, the localization of nanoparticles in cancer cells was studied. These methods have shown that nanoparticles pass through the cytoplasmic membrane and are localized inside the cells. It was also shown that nanoparticles (HA:CS-Rhod) were localized in the cytoplasm of African green monkey renal epithelial cells. It was found that the incorporation of DNIC into the composition of nanoparticles significantly increased the stability of DNIC, while prolonging the formation time and increasing the yield of nitric oxide. Thus, we have developed unique nanoparticles for the targeted delivery of antitumor drugs into cells.","PeriodicalId":9026,"journal":{"name":"Biointerface Research in Applied Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biointerface Research in Applied Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33263/briac134.344","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 0

Abstract

Cancer diseases are characterized by high incidence and mortality worldwide. The main problem in cancer therapy is the lack of specificity of anti-cancer drugs. Therefore, the development of new methods of targeted delivery of anti-cancer drugs is an urgent task in oncology. Nanoparticles from hyaluronic acid and chitosan (HA:CS) were obtained using ionotropic gelation technology. The size of the nanoparticles was investigated using dynamic light scattering. Nanoparticles were obtained of a size of 100-400 nm. A physical association method has been developed for encapsulating nanoparticles with doxorubicin, a well-known antitumor drug, and dinitrosyl iron complex (DNIC; donor of nitric oxide). Using the method of dynamic light scattering, the surface potential of nanoparticles was measured. It was found that the resulting nanoparticles (HA-DOX:CS) were stable and had a surface potential of -45.6 meV. Using the method of confocal and FLIM microscopy, the localization of nanoparticles in cancer cells was studied. These methods have shown that nanoparticles pass through the cytoplasmic membrane and are localized inside the cells. It was also shown that nanoparticles (HA:CS-Rhod) were localized in the cytoplasm of African green monkey renal epithelial cells. It was found that the incorporation of DNIC into the composition of nanoparticles significantly increased the stability of DNIC, while prolonging the formation time and increasing the yield of nitric oxide. Thus, we have developed unique nanoparticles for the targeted delivery of antitumor drugs into cells.

查看原文本刊更多论文

基于透明质酸和壳聚糖的抗癌药物靶向递送纳米粒子的胞浆内转运

癌症疾病的特点是全球发病率和死亡率高。癌症治疗的主要问题是缺乏抗癌药物的特异性。因此，开发抗癌药物靶向给药的新方法是肿瘤学领域的一项紧迫任务。采用离子凝胶技术从透明质酸和壳聚糖中制备了纳米透明质酸。使用动态光散射研究了纳米颗粒的尺寸。获得了尺寸为100-400nm的纳米颗粒。已经开发了一种用阿霉素（一种著名的抗肿瘤药物）和二硝基铁复合物（DNIC；一氧化氮供体）封装纳米颗粒的物理缔合方法。采用动态光散射法测量了纳米颗粒的表面电位。发现得到的纳米颗粒（HA-DOX:CS）是稳定的，并且具有-45.6meV的表面电势。采用共聚焦和FLIM显微镜方法研究了纳米颗粒在癌症细胞中的定位。这些方法表明，纳米颗粒穿过细胞质膜并定位在细胞内。研究还表明，纳米颗粒（HA:CS-Rhod）定位于非洲绿猴肾上皮细胞的细胞质中。研究发现，在纳米颗粒的组成中掺入DNIC显著提高了DNIC的稳定性，同时延长了形成时间并提高了一氧化氮的产率。因此，我们开发了独特的纳米颗粒，用于将抗肿瘤药物靶向递送到细胞中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biointerface Research in Applied Chemistry CHEMISTRY, APPLIED-

CiteScore

4.80

自引率

0.00%

发文量

256

期刊介绍： Biointerface Research in Applied Chemistry is an international and interdisciplinary research journal that focuses on all aspects of nanoscience, bioscience and applied chemistry. Submissions are solicited in all topical areas, ranging from basic aspects of the science materials to practical applications of such materials. With 6 issues per year, the first one published on the 15th of February of 2011, Biointerface Research in Applied Chemistry is an open-access journal, making all research results freely available online. The aim is to publish original papers, short communications as well as review papers highlighting interdisciplinary research, the potential applications of the molecules and materials in the bio-field. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible.