3D bioprinting of gene delivery scaffolds with controlled release

Q1 Computer Science

Bioprinting Pub Date : 2023-06-01 DOI:10.1016/j.bprint.2023.e00270

Yi Xiang , Zheng Zhong , Emmie J. Yao , Wisarut Kiratitanaporn , Malleeka T. Suy , Shaochen Chen

引用次数: 2

Abstract

Localized gene delivery via engineered scaffolds offers spatiotemporal control of the gene vector release. Here, we explored the capability of digital light processing based bioprinting to fabricate 3D scaffolds in hydrogels for controlled gene delivery. We demonstrated the compatibility of the method with three representative hydrogel biomaterials for gene delivery. We further investigated the highly tunable release profile with these scaffolds by creating and combining distinct release mechanisms of diffusion and ion exchange. The efficacy of gene delivery of these scaffolds was validated in vitro using 293T cells. Results from this work could potentially facilitate the development of synergistic and personalized gene therapies.

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具有控制释放的基因递送支架的3D生物打印

通过工程支架的局部基因传递提供了基因载体释放的时空控制。在这里，我们探索了基于数字光处理的生物打印技术在水凝胶中制造3D支架的能力，以控制基因的传递。我们证明了该方法与三种代表性的水凝胶生物材料在基因传递方面的相容性。我们通过创建和结合不同的扩散和离子交换释放机制，进一步研究了这些支架的高度可调释放特性。利用293T细胞体外实验验证了这些支架的基因递送效果。这项工作的结果可能会促进协同和个性化基因治疗的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioprinting Computer Science-Computer Science Applications

CiteScore

11.50

自引率

0.00%

发文量

审稿时长

68 days

期刊介绍： Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.