Inhibitory effect of high hydrostatic pressure, nisin, and moderate heating on the inactivation of Paenibacillus sp. and Terribacillus aidingensis spores isolated from UHT milk

IF 1.2 4区物理与天体物理 Q3 PHYSICS, MULTIDISCIPLINARY

High Pressure Research Pub Date : 2021-07-03 DOI:10.1080/08957959.2021.1946054

S. Kmiha, C. Modugno, C. Aouadhi, H. Simonin, S. Méjri, J. Perrier-Cornet, A. Maaroufi

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引用次数: 5

Abstract

ABSTRACT The sensitivity of Paenibacillus sp. and Terribacillus aidingensis spores to combined action of HP, nisin and moderate heating were investigated for the first time. The results showed that the effect of HP on spores varies with pressure level, treatment temperature, spore species and presence of nisin. T. aidingensis spores were more resistant than Paenibacillus sp., whatever the treatment applied. The inactivation rates of both spore species did not exceed 2.7log (CFU/mL) after HP treatment at 600 MPa/50°C/10 min. However, a high synergistic effect of nisin and HP was observed after a treatment at 500 MPa/10 min/50°C with addition of nisin during HP and into the recovery medium, leading to 6log (CFU/mL) and 4log (CFU/mL) reduction for Paenibacillus and Terribacillus spores, respectively. Finally, the fraction of germinated spores by HP was measured and the results showed that germination induction is not the only underlying mechanisms of nisin sensitization by HP.

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高静水压、乳酸链球菌肽和适度加热对超高温灭菌乳中分离的Paenibacillus sp.和Terrillus aidingensis孢子灭活的抑制作用

首次研究了Paenibacillus sp.和Terribacillus aidingensis孢子对HP、nisin和温和加热联合作用的敏感性。结果表明，HP对孢子的影响随压力水平、处理温度、孢子种类和nisin的存在而变化。无论施用何种处理，辅助芽孢杆菌孢子的抗性都强于芽孢杆菌。HP在600 MPa/50℃/10 min下处理后，两种孢子的失活率均不超过2.7log (CFU/mL)。然而，在500 MPa/10 min/50℃条件下，在HP和恢复培养基中添加nisin后，观察到nisin和HP的高度协同效应，导致Paenibacillus和Terribacillus孢子分别减少6log (CFU/mL)和4log (CFU/mL)。最后，我们测量了HP的萌发孢子的比例，结果表明萌发诱导并不是HP致敏nisin的唯一潜在机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

High Pressure Research 物理-物理：综合

CiteScore

3.80

自引率

5.00%

发文量

审稿时长

2 months

期刊介绍： High Pressure Research is the leading journal for research in high pressure science and technology. The journal publishes original full-length papers and short research reports of new developments, as well as timely review articles. It provides an important forum for the presentation of experimental and theoretical advances in high pressure science in subjects such as: condensed matter physics and chemistry geophysics and planetary physics synthesis of new materials chemical kinetics under high pressure industrial applications shockwaves in condensed matter instrumentation and techniques the application of pressure to food / biomaterials Theoretical papers of exceptionally high quality are also accepted.