The Anti-biofilm Activity of Nanometric Zinc doped Bioactive Glass against Putative Periodontal Pathogens: An in vitro Study

Abstract

Abstract Colonization of periodontal pathogens on the surgical sites is one of the primary reasons for the failure of regenerative periodontal therapies. Bioactive glasses (BGs) owing to their favorable structural and antimicrobial properties have been proposed as promising materials for the reconstruction of periodontal and peri-implant bone defects. This study aimed to investigate the antibiofilm activity of zinc-doped BG (Zn/BG) compared with 45S5 Bioglass® (BG®) on putative periodontal pathogens. In this in vitro experimental study, the nano BG doped with 5-mol% zinc and BG® were synthesized by sol-gel method. Mono-species biofilms of Aggregatibacter actinomycetemcomitans (A. a), Porphyromonas gingivalis (P. g), and Prevotella intermedia (P. i)were prepared separately in a well-containing microplate. After 48 hours of exposure to generated materials at 37°C, the anti-biofilm potential of the samples was studied by measuring the optical density (OD) at 570nm wavelengths with a microplate reader. Two-way ANOVA then analyzed the results. Both Zn/BG and BG® significantly reduced the biofilm formation ability of all examined strains after 48 hours of incubation (P=0.0001). Moreover, the anti-biofilm activity of Zn/BG was significantly stronger than BG® (P=0.0001), which resulted in the formation of a weak biofilm (OD<1) compared with a moderately adhered biofilm observed with BG® (1

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纳米锌掺杂生物活性玻璃抗牙周病病原菌生物膜活性的体外研究

摘要牙周病原体在手术部位的定植是再生牙周治疗失败的主要原因之一。生物活性玻璃由于其良好的结构和抗菌性能，已被认为是重建牙周和种植体周围骨缺损的有前途的材料。本研究旨在研究锌掺杂BG（Zn/BG）与45S5 Bioglass®（BG®）对假定牙周病原体的抗菌膜活性。在本体外实验研究中，采用溶胶-凝胶法合成了掺杂5-mol%锌的纳米BG和BG®。在含有微孔板的井中分别制备了聚合放线菌（A.A）、牙龈卟啉单胞菌（P.g）和中间普雷沃氏菌（P.i）的单物种生物膜。在37°C下暴露于生成的材料48小时后，通过用微孔板读取器测量570nm波长下的光密度（OD）来研究样品的抗生物膜潜力。然后进行双向方差分析。培养48小时后，Zn/BG和BG®均显著降低了所有受试菌株的生物膜形成能力（P=0.0001）。此外，Zn/BG的抗生物膜活性显著强于BG®（P=0.0001。Zn/BG对所有检测的牙周病原体的生物膜形成都显示出显著的抑制作用。鉴于这种新型生物材料具有增强的再生和抗生物膜特性，需要进一步研究才能在临床应用中实施。

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