Changes and significance of serum Klotho protein levels in different stages of chronic kidney disease

中国综合临床 Pub Date : 2019-11-01 DOI:10.3760/CMA.J.ISSN.1008-6315.2019.06.013

Aiqin Liu, Lei Yu, Lige Zhu, De-ting Zhang

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Abstract

Objective To explore the changes and significance of Klotho protein levels in different stages of chronic kidney disease (CKD). Methods From March 2015 to December 2017, 176 patients with CKD admitted to nephrology department of Inner Mongolia People's Hospital were selected as the study object (CKD group), and 80 healthy patients in our hospital were selected as the control group in the same period.The serum Klotho levels of CKD patients and control group at different stages were detected by double antibody sandwich ELISA, and the differences between each group were compared. Results The serum Klotho level of CKD group ((4.84±1.87) μg/L) was significantly lower than that of the control group ((9.11±3.14) μg/L)(t=13.82, P<0.01). One-way anova showed that estimated renal glomerular filtration rate (eGFR), serum albumin (ALB), hemoglobin (Hb), blood calcium (Ca) and serum Klotho were gradually decreased, while phosphorus (P) and creatinine (Cr) in serum were gradually increased, and the difference was statistically significant among the five stages(all P<0.01). Spearman correlation analysis showed that Klotho level was positively correlated with eGFR and Ca, and negatively correlated with CKD stage, Cr and P (r=0.369, 0.160, -0.200, -0.250, -0.230, all P<0.05). The multiple linear regression equation showed that Klotho level was positively and independently correlated with eGFR (t=3.89, P<0.001), and negatively correlated with CKD staging independently (t=-4.12, P<0.001). Conclusion The expression level of serum Klotho protein in patients with CKD is lower than that of healthy people, and it decreases with the increase of CKD stages, which is closely related to the deterioration of renal function.It can be used as a reference index to evaluate the incidence and severity of CKD. Key words: Chronic kidney disease; Klotho protein; Chronic kidney disease stage; Estimated glomerular filtration rate; Renal function

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慢性肾脏病不同阶段血清Klotho蛋白水平的变化及其意义

目的探讨慢性肾脏病（CKD）不同阶段Klotho蛋白水平的变化及其意义。方法选择2015年3月至2017年12月入住内蒙古人民医院肾内科的176例CKD患者作为研究对象（CKD组），同期选择我院80例健康患者作为对照组。采用双抗体夹心ELISA法检测CKD患者和对照组不同阶段血清Klotho水平，并比较各组之间的差异。结果CKD组血清Klotho水平（4.84±1.87）μg/L）明显低于对照组（9.11±3.14）μg/L（t=13.82，P<0.01），单向方差分析显示：肾小球滤过率（eGFR）、血清白蛋白（ALB）、血红蛋白（Hb）、血钙（Ca）、血清Kloth逐渐降低，血清磷（P）和肌酐（Cr）逐渐升高，五个阶段之间差异有统计学意义（均P<0.01）。Spearman相关分析显示，Klotho水平与eGFR和Ca呈正相关，与CKD分期呈负相关，Cr和P（r=0.369，0.160，-0.200，-0.250，-0.230，均P<0.05）。多元线性回归方程显示，Klotho水平与eGFR呈正独立相关（t=3.89，P<0.001），与CKD分期独立负相关（t=-4.12，P<0.001，并且随着CKD分期的增加而降低，这与肾功能的恶化密切相关。可作为评估CKD发病率和严重程度的参考指标。关键词：慢性肾脏病；Klotho蛋白；慢性肾脏病阶段；估计肾小球滤过率；肾功能

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来源期刊

中国综合临床

CiteScore

0.10

自引率

0.00%

发文量

16855

期刊介绍： Clinical Medicine of China is an academic journal organized by the Chinese Medical Association (CMA), which mainly publishes original research papers, reviews and commentaries in the field. Clinical Medicine of China is a source journal of Peking University (2000 and 2004 editions), a core journal of Chinese science and technology, an academic journal of RCCSE China Core (Extended Edition), and has been published in Chemical Abstracts of the United States (CA), Abstracts Journal of Russia (AJ), Chinese Core Journals (Selection) Database, Chinese Science and Technology Materials Directory, Wanfang Database, China Academic Journal Database, JST Japan Science and Technology Agency Database (Japanese) (2018) and other databases.