Phytochemical Analysis and in Vitro Assessment of Himalayan Polystichum squarrosum Extract for Its Antioxidant and Antimicrobial Activities

IF 1.2 Q4 PHARMACOLOGY & PHARMACY

Journal of Herbs, Spices and Medicinal Plants Pub Date : 2022-05-23 DOI:10.1080/10496475.2022.2078919

K. Khulbe, Poonam Takuli, Parikshit Kumar, Archana Parki

引用次数: 1

Abstract

ABSTRACT Aerial parts of Polystichum squarrosum (Dryopteridaceae) collected from Kumaun Himalaya, India were assessed for antioxidant potential using the methanolic extract in vitro for DPPH free radical scavenging and ferric-reducing antioxidant assays. The DPPH assay exhibited potent free radical scavenging capacity (IC50, 6.03 ± 1.41 µg mL−1), as well as ferric-reducing antioxidant activity (886.2 mg AAE gm−1 dry extract). In the antibacterial activity tests, the methanolic extract inhibited the growth of Klebsiella pneumoniae (MIC 14 ± 0.57 mm) and Agrobacterium tumefaciens (MIC 12 ± 0.7 mm). Totally, 65 phytochemical compounds were identified using gas chromatography mass spectrometry (GC-MS). Total phenolics (913.33 ± 39.23 mg GAE g−1 dry extract) and total flavonoid contents (30.0 ± 2.64 mg QE g−1 dry extract) were estimated qualitatively. These results demonstrated antioxidant and antimicrobial activities due to the presence of bioactive phytochemicals.

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喜马拉雅多刺荆提取物抗氧化和抗菌活性的植物化学分析及体外评价

摘要利用甲醇提取物对从印度库马恩喜马拉雅山采集的鳞毛蕨（Dryopteridaceae）的地上部分进行DPPH自由基清除和铁还原抗氧化试验，评估了其抗氧化潜力。DPPH测定显示出强大的自由基清除能力（IC50，6.03±1.41µg mL−1），以及铁还原抗氧化活性（886.2 mg AAE gm−1干提取物）。在抗菌活性测试中，甲醇提取物抑制肺炎克雷伯菌（MIC 14±0.57mm）和根癌农杆菌（MIC 12±0.7mm）的生长。采用气相色谱-质谱联用技术共鉴定出65种植物化学成分。对总酚（913.33±39.23 mg GAE g−1干提取物）和总黄酮含量（30.0±2.64 mg QE g−1干燥提取物）进行了定性估算。这些结果表明，由于存在生物活性植物化学物质，具有抗氧化和抗菌活性。

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来源期刊

Journal of Herbs, Spices and Medicinal Plants Medicine-Complementary and Alternative Medicine

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.