Lipidomic Markers of Tumor Progress in Breast Cancer Patients

IF 0.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Pub Date : 2022-08-15 DOI:10.1134/S1990750822030118

A. O. Tokareva, N. L. Starodubtseva, V. V. Chagovets, V. V. Rodionov, V. V. Kometova, K. S. Chingin, V. E. Frankevich

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引用次数: 1

Abstract—

Research of cancer progression mechanisms and their impact on metabolism of tumor cells and tumor microenvironment cells is an important element in drug development for cancer target therapy. In this study, changes in tumor tissue and margin tissue lipid profiles were investigated for their association with the following clinical and morphological characteristics: tumor size, cancer stage, multifocality, tumor grade, number of lymph node metastases, Nottingham prognostic index, total malignancy score, and the level of Ki67 protein. Lipid profiling was performed by reverse-phase chromato-mass spectrometry analysis of the lipid tissue extract with lipid identification by characteristic fragments. In the lipid profile of tumor tissue 13 characteristic lipids were selected. Their levels significantly correlated with at least 5 clinical and morphological features. Eight of 13 lipid features belonged to phosphatidylcholines. In the lipid profile of tumor microenvironment tissue, 13 lipid features were selected. Their levels significantly correlated with at least 5 clinical and morphological features. Four of 13 lipid features belonged to oxidized lipids, 4 lipid features belonged to sphingomyelins, 4 lipid features belonged to phosphatidylethanolamines. The tumor microenvironment tissue lipid profile correlated with the tumor size, the cancer stage, the tumor grade, the number of axillary metastases, and the Nottingham prognostic index. The tumor tissue lipid profile correlated with tumor size, tumor grade, total malignant score, and number of axillary metastases.

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乳腺癌患者肿瘤进展的脂质组学标志物

摘要研究肿瘤进展机制及其对肿瘤细胞和肿瘤微环境细胞代谢的影响是肿瘤靶向治疗药物开发的重要内容。在本研究中，我们研究了肿瘤组织和边缘组织脂质谱的变化与以下临床和形态学特征的关系:肿瘤大小、肿瘤分期、多灶性、肿瘤分级、淋巴结转移数量、诺丁汉预后指数、恶性肿瘤总评分和Ki67蛋白水平。脂质分析采用反相质谱法对脂质组织提取物进行分析，并通过特征片段进行脂质鉴定。在肿瘤组织脂质谱中选取了13种特征脂质。它们的水平与至少5个临床和形态学特征显著相关。13个脂质特征中有8个属于磷脂酰胆碱。在肿瘤微环境组织的脂质谱中，选取了13个脂质特征。它们的水平与至少5个临床和形态学特征显著相关。13个脂质特征中有4个属于氧化脂质，4个属于鞘磷脂，4个属于磷脂酰乙醇胺。肿瘤微环境组织脂质谱与肿瘤大小、肿瘤分期、肿瘤分级、腋窝转移数、诺丁汉预后指数相关。肿瘤组织脂质谱与肿瘤大小、肿瘤分级、总恶性评分和腋窝转移数相关。

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来源期刊

Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry covers all major aspects of biomedical chemistry and related areas, including proteomics and molecular biology of (patho)physiological processes, biochemistry, neurochemistry, immunochemistry and clinical chemistry, bioinformatics, gene therapy, drug design and delivery, biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine. The journal also publishes review articles. All issues of the journal usually contain solicited reviews.