Nematicidal effect of an Amaranthus hypochondriacus L. cystatin (AhCPI) on the root-knot nematode Meloidogyne incognita (Kofoid and White) Chitwood

IF 2.2 3区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY
María Magdalena Cervantes-Juan, Jorge E. Ibarra, Víctor Olalde-Portugal, María Karina Manzo-Valencia, Marco Antonio Meraz Mercado, Silvia Valdés-Rodríguez
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Abstract

The root-knot nematode Meloidogyne incognita is one of the most damaging plant parasitic nematodes in the world. In this study, the effect of cystatin from Amaranthus hypochondriacus (AhCPI) as a potential control agent for M. incognita was explored. In vitro bioassays demonstrated that AhCPI affects the growth and development of eggs and the infectivity of juveniles (J2) of M. incognita, such as mortality and slower development, showing characteristic tissue damage. Mortality levels were quantified by Probit analysis, estimating LC50s of 1.4 mg/mL for eggs and 0.028 mg/mL for J2. In planta bioassays showed that infected tomato seedlings treated with 0.056 mg/mL of AhCPI showed a 60% reduction in the number of galls, as compared with untreated J2-inoculated seedlings. Under greenhouse conditions, three applications of 10 mL of AhCPI (1.4 mg/mL) in the soil around the stem of M. incognita-infected tomato plants, reduced the number of galls by 93 ± 8%, as compared to the control M. incognita-infected plants. The application of AhCPI to the infected plants increased the yield (10.7%) of harvested tomato fruits, as compared to infected plants. These results show the potential of AhCPI for the control of M. incognita in tomato plants.

Abstract Image

Amaranthus hypochodiacus L.cystatin(AhCPI)对根结线虫南方根结线虫(Kofoid and White)Chitwood的杀线虫作用
根结线虫南方根结线虫是世界上危害最大的植物寄生线虫之一。在本研究中,探讨了Amaranthus hypochodiacus(AhCPI)的胱抑素作为隐姓埋名M.incognita的潜在控制剂的作用。体外生物测定表明,AhCPI会影响隐翅虫卵的生长发育和幼体(J2)的传染性,如死亡率和发育迟缓,表现出特征性的组织损伤。死亡率水平通过Probit分析进行量化,估计LC50为1.4 鸡蛋为mg/mL,0.028 对于J2为mg/mL。植物体内生物测定表明,用0.056处理的受感染番茄幼苗 mg/mL的AhCPI显示与未处理的接种J2的幼苗相比,胆囊数量减少了60%。在温室条件下,10 mL AhCPI(1.4 mg/mL)在感染了M.incognita的番茄植株茎部周围的土壤中,使胆结石的数量减少了93 ± 8%。与感染植物相比,将AhCPI应用于感染植物可提高收获番茄果实的产量(10.7%)。这些结果显示了AhCPI在番茄植株中控制隐翅虫的潜力。
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来源期刊
Annals of Applied Biology
Annals of Applied Biology 生物-农业综合
CiteScore
5.50
自引率
0.00%
发文量
71
审稿时长
18-36 weeks
期刊介绍: Annals of Applied Biology is an international journal sponsored by the Association of Applied Biologists. The journal publishes original research papers on all aspects of applied research on crop production, crop protection and the cropping ecosystem. The journal is published both online and in six printed issues per year. Annals papers must contribute substantially to the advancement of knowledge and may, among others, encompass the scientific disciplines of: Agronomy Agrometeorology Agrienvironmental sciences Applied genomics Applied metabolomics Applied proteomics Biodiversity Biological control Climate change Crop ecology Entomology Genetic manipulation Molecular biology Mycology Nematology Pests Plant pathology Plant breeding & genetics Plant physiology Post harvest biology Soil science Statistics Virology Weed biology Annals also welcomes reviews of interest in these subject areas. Reviews should be critical surveys of the field and offer new insights. All papers are subject to peer review. Papers must usually contribute substantially to the advancement of knowledge in applied biology but short papers discussing techniques or substantiated results, and reviews of current knowledge of interest to applied biologists will be considered for publication. Papers or reviews must not be offered to any other journal for prior or simultaneous publication and normally average seven printed pages.
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