Morphological and molecular characterisation of Trachactinolaimus nanjingensis n. sp. (Dorylaimida: Actinolaimidae) from Nanjing, China

IF 1.2 4区生物学 Q2 ZOOLOGY

Nematology Pub Date : 2023-02-01 DOI:10.1163/15685411-bja10222

Qi Zhang, H. Ji, F. Guo, Xue Qing, Hongmei Li

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引用次数: 0

Abstract

Trachactinolaimus nanjingensis n. sp. isolated from mosses at the Yangshan Quarry Park in Nanjing, P.R. China, is described. It is characterised by 3.01 (2.64-3.42) mm long female and 2.84 (2.50-3.22) mm long male, four onchia with numerous mural denticles (>100), vulva with a transverse slit, 15-19 contiguous ventromedian supplements, spicules length of 61.5 (54.6-64.3) μm, and female and male both having long tail, with c′ values equal to 5.0-9.2 and 2.3-3.5, respectively. The new species was morphologically compared with T. brevicaudatus, T. dominicus, T. montanus and T. radulatus. The sequences of 18S and 28S rRNA genes of the new species were obtained using regular PCR amplification, while the ITS and mtCOI sequences were extracted from the whole genome sequencing assembly. The phylogenetic relationships of the new species were reconstructed using 18S and 28S sequences and the status of the new species was discussed. The 18S and 28S sequences of a population of genus Westindicus recovered during the present study were also obtained. This is the first time to present the ITS and mtCOI sequences of family Actinolaimidae.

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南京放线虫的形态和分子特征(多线虫目:放线虫科)

介绍了从南京阳山采石场的苔藓中分离得到的南京Trachactinolaimus nanjingensis n.sp。其特征是雌性长3.01（2.64-3.42）mm，雄性长2.84（2.50-3.22。将该新种与短尾锥虫、多米尼库斯锥虫、山地锥虫和拉杜拉锥虫进行形态比较。利用常规PCR扩增获得新物种的18S和28S rRNA基因序列，同时从全基因组测序组件中提取ITS和mtCOI序列。利用18S和28S序列重建了新种的系统发育关系，并对新种的地位进行了讨论。还获得了在本研究中回收的Westindicus属种群的18S和28S序列。这是首次提出放线菌科的ITS和mtCOI序列。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nematology 生物-动物学

CiteScore

2.60

自引率

33.30%

发文量

审稿时长

3 months

期刊介绍： Nematology is an international journal for the publication of all aspects of nematological research (with the exception of vertebrate parasitology), from molecular biology to field studies. Papers on nematode parasites of arthropods, and on soil free-living nematodes, and on interactions of these and other organisms, are particularly welcome. Research on fresh water and marine nematodes is also considered when the observations are of more general interest. Nematology publishes full research papers, short communications, Forum articles (which permit an author to express a view on current or fundamental subjects), perspectives on nematology, and reviews of books and other media.