Oral administration of lithium chloride ameliorate spinal cord injury-induced hyperalgesia in male rats

IF 2.4 Q3 NUTRITION & DIETETICS
Golnoosh Rahimi , Sara Mirsadeghi , Saeid Rahmani , Amin Izadi , Zahra Ghodsi , Seyed Mohammad Ghodsi , Vafa Rahimi-Movaghar , Sahar Kiani
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引用次数: 0

Abstract

Background

Numerous studies have described the neuroprotective effect of lithium in spinal cord injury in addition to its ameliorative impact on pain sensation. In the present study, we aim to examine the efficacy of 85 mg/kg as well as 50 mg/kg dosage of the lithium chloride (LiCl) through oral consumption in spinal cord injured rats and their effect on gene expression of three candidate genes, corresponding to the hyper-sensitization.

Methods

Adult Wistar (male) rats were divided into four experimental groups: control; oral administration of LiCl with 85 mg/kg and 50 mg/kg dosage; and 10 % sucrose receiver as the vehicle. BBB and heat plantar tests were performed weekly throughout four weeks to evaluate motor improvement and neuropathic pain amelioration, i.e., the alleviation in hyperalgesia. Then, the expression pattern of Kcnd2, ERK and Gria2 genes were assessed.

Results

The BBB results demonstrated that LiCl with both dosages does not allow remarkable improvement in motor function during four weeks of treatment. The heat plantar tests show substantial recovery in LiCl treated groups versus vehicle and control after four weeks of evaluation. According to Real-time PCR, Kcnd2 and Gria2 were up-regulated in the presence of lithium in a dose-dependent manner while ERK expression was not differed remarkably.

Conclusion

Our results suggested that LiCl allows hyperalgesia palliation, however, did not reinforce persistent motor improvement. Also, oral lithium consumption with 50 mg/kg concentration, entails considerable restoration in gene expression level of Kcnd2 and Gria2.

口服氯化锂改善雄性大鼠脊髓损伤性痛觉过敏
大量研究已经描述了锂在脊髓损伤中的神经保护作用,以及它对疼痛感觉的改善作用。在本研究中,我们旨在通过口服85 mg/kg和50 mg/kg剂量的氯化锂(LiCl)对脊髓损伤大鼠的作用,以及它们对三种与超致敏相关的候选基因表达的影响。方法成年Wistar(雄性)大鼠分为4个实验组:对照组;口服LiCl,剂量分别为85 mg/kg和50 mg/kg;10%蔗糖受体作为载体。每周进行BBB和足底热测试,持续四周,以评估运动改善和神经性疼痛改善,即痛觉过敏的缓解。然后,检测kcn2、ERK和Gria2基因的表达谱。结果血脑屏障结果显示,在4周的治疗期间,两种剂量的LiCl对运动功能没有显著改善。足底热测试显示,经过四周的评估,与对照和对照相比,LiCl处理组的足底热恢复明显。Real-time PCR结果显示,锂离子存在时,kcn2和Gria2呈剂量依赖性上调,而ERK的表达无显著差异。结论:LiCl可缓解痛觉过敏,但不能增强持续性运动改善。此外,口服50 mg/kg浓度的锂,可显著恢复kcn2和Gria2的基因表达水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
PharmaNutrition
PharmaNutrition Agricultural and Biological Sciences-Food Science
CiteScore
5.70
自引率
3.10%
发文量
33
审稿时长
12 days
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