Development of an efficient dispersive liquid-liquid microextraction approach combined with spectrophotometry for determination of antiviral drug, valacyclovir HCl in pharmaceutical formulations

IF 1.3 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Bulletin of the Chemical Society of Ethiopia Pub Date : 2023-03-07 DOI:10.4314/bcse.v37i3.4

Amal H. Al-Bagawi, Samya Sh. Alenezi, Ragaa El Sheikh, Ghada M. ABDEL FATTAH, Ayman A. Gouda

{"title":"Development of an efficient dispersive liquid-liquid microextraction approach combined with spectrophotometry for determination of antiviral drug, valacyclovir HCl in pharmaceutical formulations","authors":"Amal H. Al-Bagawi, Samya Sh. Alenezi, Ragaa El Sheikh, Ghada M. ABDEL FATTAH, Ayman A. Gouda","doi":"10.4314/bcse.v37i3.4","DOIUrl":null,"url":null,"abstract":"ABSTRACT. A new, fast, sensitive, simple, and green dispersive liquid–liquid microextraction (DLLME)-based spectrophotometric method for the determination of valacyclovir HCl (VAL) in pure form and pharmaceutical formulations has been developed and validated. The developed method is based on the formation of a coloured product through the reaction of VAL and 1,2-naphthoquine-4-sulfonate (NQS) at pH = 9. The important experimental parameters affecting the extraction efficiency were investigated and optimized. The tiny organic droplets had a wavelength of l = 505 nm. At the optimum conditions, linearity ranged from 0.06 to 2.0 μg/mL, with a linear correlation coefficient of 0.9995. The limits of detection and quantification were 0.02, and 0.06 μg/mL, respectively. The enhancement factor was 36.87. Good recovery as accuracy (99.50%) and relative standard deviation (RSD) as precision were 1.20%, respectively. The developed DLLME method was successfully applied to the determination of VAL in pharmaceutical formulations, and the validity was assessed by applying the standard addition technique. The results obtained by the proposed method for the pure VAL and commercial tablets agreed well with those obtained by the official method. \n \nKEY WORDS: Valacyclovir HCl, Spectrophotometry, 1-2-Naphthoquine-4-sulfonate, Dispersive liquid–liquid microextraction, Pharmaceutical formulations \n \nBull. Chem. Soc. Ethiop. 2023, 37(3), 579-592. \nDOI: https://dx.doi.org/10.4314/bcse.v37i3.4","PeriodicalId":9501,"journal":{"name":"Bulletin of the Chemical Society of Ethiopia","volume":" ","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2023-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of the Chemical Society of Ethiopia","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.4314/bcse.v37i3.4","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

ABSTRACT. A new, fast, sensitive, simple, and green dispersive liquid–liquid microextraction (DLLME)-based spectrophotometric method for the determination of valacyclovir HCl (VAL) in pure form and pharmaceutical formulations has been developed and validated. The developed method is based on the formation of a coloured product through the reaction of VAL and 1,2-naphthoquine-4-sulfonate (NQS) at pH = 9. The important experimental parameters affecting the extraction efficiency were investigated and optimized. The tiny organic droplets had a wavelength of l = 505 nm. At the optimum conditions, linearity ranged from 0.06 to 2.0 μg/mL, with a linear correlation coefficient of 0.9995. The limits of detection and quantification were 0.02, and 0.06 μg/mL, respectively. The enhancement factor was 36.87. Good recovery as accuracy (99.50%) and relative standard deviation (RSD) as precision were 1.20%, respectively. The developed DLLME method was successfully applied to the determination of VAL in pharmaceutical formulations, and the validity was assessed by applying the standard addition technique. The results obtained by the proposed method for the pure VAL and commercial tablets agreed well with those obtained by the official method. KEY WORDS: Valacyclovir HCl, Spectrophotometry, 1-2-Naphthoquine-4-sulfonate, Dispersive liquid–liquid microextraction, Pharmaceutical formulations Bull. Chem. Soc. Ethiop. 2023, 37(3), 579-592. DOI: https://dx.doi.org/10.4314/bcse.v37i3.4

查看原文本刊更多论文

建立高效分散液液微萃取联合分光光度法测定制剂中抗病毒药物盐酸伐昔洛韦的方法

摘要。开发并验证了一种新的、快速、灵敏、简单、绿色分散的基于液-液微萃取（DLLME）的分光光度法测定纯形式和药物制剂中盐酸缬昔洛韦（VAL）的方法。所开发的方法基于在pH＝9下通过VAL和1，2-萘醌-4-磺酸酯（NQS）的反应形成有色产物。对影响萃取效率的重要实验参数进行了研究和优化。微小的有机液滴具有l＝505nm的波长。在最佳条件下，线性范围为0.06-2.0μg/mL，线性相关系数为0.9995。检测限和定量限分别为0.02和0.06μg/mL。增强因子为36.87。准确度的良好回收率（99.50%）和精密度的相对标准偏差（RSD）分别为1.20%。将所开发的DLLME方法成功地应用于药物制剂中缬氨酸的测定，并应用标准加入技术对其有效性进行了评估。通过所提出的方法获得的纯缬氨酸和市售片剂的结果与通过官方方法获得的结果一致。关键词：盐酸伐昔洛韦，分光光度法，1-2-萘醌-4-磺酸盐，分散液-液微萃取，药物配方Bull。化学。Soc.Ethiop。2023，37（3），579-592。DOI:https://dx.doi.org/10.4314/bcse.v37i3.4

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bulletin of the Chemical Society of Ethiopia 化学-化学综合

CiteScore

2.20

自引率

8.30%

发文量

113

审稿时长

6-12 weeks

期刊介绍： The Bulletin of the Chemical Society of Ethiopia (BCSE) is a triannual publication of the Chemical Society of Ethiopia. The BCSE is an open access and peer reviewed journal. The BCSE invites contributions in any field of basic and applied chemistry.