The Inhibitory effect of hydroalcoholic extract from the Algerian Echium trygorrhizum Pomel roots on α-amylase activity.

Q4 Pharmacology, Toxicology and Pharmaceutics

Current Enzyme Inhibition Pub Date : 2021-12-24 DOI:10.2174/1573408018666211224092636

Allaoua Nouri, Lakhder Gasmi, Chawki Bensebini, D. Harzallah, S. Khennouf, S. Dahamna

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Abstract

Species of Echium trygorrhizum Pomel , are used traditionally in Algeria folk medicine for the treatment of Diabetes, Jaundice and Tonsillitis. To our knowledge, no previous study has been conducted out on the pharmacological activities of this species. The objective of the present research was to evaluate the content of polyphenols , flavonoids and condensed tannins compounds and to assess in vitro the antioxidant activity and the inhibitory effect of the hydroalcoholic extract of this plant, on α-amylase activity, an enzyme responsible for digestion of carbohydrate before the process of intestinal absorption. Polyphenols, flavonoids and condensed tannins were evaluated spectrophotometrically using Folin–Ciocalteu, the Aluminum chloride and vanillin methods respectively. The antioxidant activity using DPPH radical scavenging, ABTS, Ferric reducing antioxidant power and β- carotene bleaching tests and the assessment of in vitro α- amylase inhibitory potential by an Aspergillus oryzae α- amylase have been studied. The hydroalcoholic crude extract was able to inhibit the α-amylase enzyme in vitro, with an IC50 of 0.56 ± 0.044 mg/ml, in addition the contents of polyphenols and flavonoids were found to be 157.403 ± 0.835 µg GAE/mg extract and 30.156±2.634µg Q E / mg extract, respectively, Whereas the amount of tannins was 65.293 ± 0.883µg Cat E/ mg of dry extract. The present study revealed that the extract is rich in phenolic compounds, which play a really important role in the scavenging of free radicals. It has been reported the inhibitory capacity of hydroalcoholic roots extract on Aspergillus oryzae α-amylase enzyme might be used as a natural agent within the management of diabetes mellitus.

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阿尔及利亚刺槐柚根水醇提取物对α-淀粉酶活性的抑制作用。

紫锥菊品种，在阿尔及利亚传统民间医学用于治疗糖尿病，黄疸和扁桃体炎。据我们所知，以前没有对该物种的药理活性进行过研究。本研究的目的是评价该植物的多酚、黄酮类化合物和缩合单宁化合物的含量，并评估其体外抗氧化活性和对α-淀粉酶活性的抑制作用，α-淀粉酶是在肠道吸收过程中负责消化碳水化合物的酶。分别用福林- ciocalteu法、氯化铝法和香兰素法测定多酚类、黄酮类和缩合单宁。采用DPPH自由基清除试验、ABTS试验、铁还原抗氧化能力试验和β-胡萝卜素漂白试验，研究了米曲霉α-淀粉酶体外抑制α-淀粉酶的活性。水醇粗提物对α-淀粉酶具有体外抑制作用，IC50值为0.56±0.044 mg/ml，其中多酚和黄酮类化合物含量分别为157.403±0.835µg GAE/mg和30.156±2.634µg Q E/mg，单宁含量为65.293±0.883µg Cat E/mg。本研究表明，该提取物含有丰富的酚类化合物，对清除自由基具有重要作用。据报道，水醇根提取物对米曲霉α-淀粉酶的抑制作用可作为治疗糖尿病的天然药物。

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来源期刊

Current Enzyme Inhibition Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： Current Enzyme Inhibition aims to publish all the latest and outstanding developments in enzyme inhibition studies with regards to the mechanisms of inhibitory processes of enzymes, recognition of active sites, and the discovery of agonists and antagonists, leading to the design and development of new drugs of significant therapeutic value. Each issue contains a series of timely, in-depth reviews written by leaders in the field, covering a range of enzymes that can be exploited for drug development. Current Enzyme Inhibition is an essential journal for every pharmaceutical and medicinal chemist who wishes to have up-to-date knowledge about each and every development in the study of enzyme inhibition.