Antonio Lozano-León , Rafael R. Rodríguez-Souto , Narjol González-Escalona , José Llovo-Taboada , José Iglesias-Canle , Ana Álvarez-Castro , Alejandro Garrido-Maestu
{"title":"Detection, molecular characterization, and antimicrobial susceptibility, of Campylobacter spp. isolated from shellfish","authors":"Antonio Lozano-León , Rafael R. Rodríguez-Souto , Narjol González-Escalona , José Llovo-Taboada , José Iglesias-Canle , Ana Álvarez-Castro , Alejandro Garrido-Maestu","doi":"10.1016/j.mran.2021.100176","DOIUrl":null,"url":null,"abstract":"<div><p>Campylobacteriosis is one of the most important reported zoonosis worldwide. Besides poultry other sources of infection have been described. In the current study, the incidence of <em>Campylobacter</em> spp. was assessed over a five-month period in mussel samples harvested from one of the most important producing areas (Galicia, NW Spain) in Europe. Out of 91 samples screened, 8% were positive and identified as <em>C. lari</em> by MALDI-TOF and whole genome sequencing. All were detected during the colder months (February and March). The antimicrobial resistance and virulence genes analysis indicated that all were multi-resistant to at least 4 antimicrobials. They were negative for the presence of 5 virulence-related genes. This is the first report of this zoonotic pathogen in mussels from one of the most important shellfish producing regions in Europe. The genomes of these 7 <em>C. lari</em> isolates were released to the genome public database at NCBI.</p></div>","PeriodicalId":48593,"journal":{"name":"Microbial Risk Analysis","volume":"18 ","pages":"Article 100176"},"PeriodicalIF":3.0000,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mran.2021.100176","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbial Risk Analysis","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352352221000189","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 4
Abstract
Campylobacteriosis is one of the most important reported zoonosis worldwide. Besides poultry other sources of infection have been described. In the current study, the incidence of Campylobacter spp. was assessed over a five-month period in mussel samples harvested from one of the most important producing areas (Galicia, NW Spain) in Europe. Out of 91 samples screened, 8% were positive and identified as C. lari by MALDI-TOF and whole genome sequencing. All were detected during the colder months (February and March). The antimicrobial resistance and virulence genes analysis indicated that all were multi-resistant to at least 4 antimicrobials. They were negative for the presence of 5 virulence-related genes. This is the first report of this zoonotic pathogen in mussels from one of the most important shellfish producing regions in Europe. The genomes of these 7 C. lari isolates were released to the genome public database at NCBI.
期刊介绍:
The journal Microbial Risk Analysis accepts articles dealing with the study of risk analysis applied to microbial hazards. Manuscripts should at least cover any of the components of risk assessment (risk characterization, exposure assessment, etc.), risk management and/or risk communication in any microbiology field (clinical, environmental, food, veterinary, etc.). This journal also accepts article dealing with predictive microbiology, quantitative microbial ecology, mathematical modeling, risk studies applied to microbial ecology, quantitative microbiology for epidemiological studies, statistical methods applied to microbiology, and laws and regulatory policies aimed at lessening the risk of microbial hazards. Work focusing on risk studies of viruses, parasites, microbial toxins, antimicrobial resistant organisms, genetically modified organisms (GMOs), and recombinant DNA products are also acceptable.