Detection, molecular characterization, and antimicrobial susceptibility, of Campylobacter spp. isolated from shellfish

IF 3 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Microbial Risk Analysis Pub Date : 2021-08-01 DOI:10.1016/j.mran.2021.100176

Antonio Lozano-León , Rafael R. Rodríguez-Souto , Narjol González-Escalona , José Llovo-Taboada , José Iglesias-Canle , Ana Álvarez-Castro , Alejandro Garrido-Maestu

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引用次数: 4

Abstract

Campylobacteriosis is one of the most important reported zoonosis worldwide. Besides poultry other sources of infection have been described. In the current study, the incidence of Campylobacter spp. was assessed over a five-month period in mussel samples harvested from one of the most important producing areas (Galicia, NW Spain) in Europe. Out of 91 samples screened, 8% were positive and identified as C. lari by MALDI-TOF and whole genome sequencing. All were detected during the colder months (February and March). The antimicrobial resistance and virulence genes analysis indicated that all were multi-resistant to at least 4 antimicrobials. They were negative for the presence of 5 virulence-related genes. This is the first report of this zoonotic pathogen in mussels from one of the most important shellfish producing regions in Europe. The genomes of these 7 C. lari isolates were released to the genome public database at NCBI.

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贝类弯曲杆菌的检测、分子特性和抗菌敏感性

弯曲杆菌病是世界上最重要的人畜共患病之一。除家禽外，还描述了其他感染源。在目前的研究中，对从欧洲最重要的产地之一(西班牙西北部的加利西亚)采集的贻贝样本进行了为期5个月的弯曲杆菌发病率评估。经MALDI-TOF和全基因组测序，筛选的91份样本中，有8%呈阳性，鉴定为拉里梭菌。所有病例均在较冷的月份(2月和3月)检测到。耐药性和毒力基因分析表明，所有菌株均对至少4种抗菌素多重耐药。5个毒力相关基因均为阴性。这是在欧洲最重要的贝类产区之一的贻贝中首次报道这种人畜共患病原体。这7个菌株的基因组被发布到NCBI的基因组公共数据库中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial Risk Analysis Medicine-Microbiology (medical)

CiteScore

5.70

自引率

7.10%

发文量

审稿时长

52 days

期刊介绍： The journal Microbial Risk Analysis accepts articles dealing with the study of risk analysis applied to microbial hazards. Manuscripts should at least cover any of the components of risk assessment (risk characterization, exposure assessment, etc.), risk management and/or risk communication in any microbiology field (clinical, environmental, food, veterinary, etc.). This journal also accepts article dealing with predictive microbiology, quantitative microbial ecology, mathematical modeling, risk studies applied to microbial ecology, quantitative microbiology for epidemiological studies, statistical methods applied to microbiology, and laws and regulatory policies aimed at lessening the risk of microbial hazards. Work focusing on risk studies of viruses, parasites, microbial toxins, antimicrobial resistant organisms, genetically modified organisms (GMOs), and recombinant DNA products are also acceptable.