Electron microscopic study on the effect of chronic fluoxetine treatment on pituitary gland and the possible therapeutic effect of adipose-derived mesenchymal stem cells in adult male albino rats

IF 1.1 4区医学 Q4 MICROSCOPY

Ultrastructural Pathology Pub Date : 2022-06-13 DOI:10.1080/01913123.2022.2083279

Nashwa S. Wahba, Assmaa O Seliem, Enas G Abd Allah, Maha Z. Mohammed

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引用次数: 0

Abstract

ABSTRACT Background: Adipose-derived mesenchymal stem cells (ADSCs) have therapeutic potential for the treatment of a variety of disorders due to their self-renewal and multipotential differentiation capabilities. Aim of the work: This study was planned to demonstrate the electron microscopic structure of the pituitary gland after chronic fluoxetine treatment and the possible therapeutic effect of ADSCs. Materials and methods:Thirty healthy male adult albino rats were classified into Control group (Group I). Fluoxetine treated (Group II) received 24 mg/kg/day of fluoxetine dissolved in 1.0 mL of tap water once a day. Fluoxetine group treated with ADSCs (Group III) received fluoxetine as group (II) for 30 days and then was injected once by ADSCs at a dose of 1 × 106 cells/rat in the tail vein suspended in 0.5 ml of phosphate-buffered saline (PBS). Recovery group (Group IV) received fluoxetine for 30 days and then received no treatment till the end of the experiment. Results: The ultrastructural observations of the fluoxetine-treated group revealed major histological changes in both the pars distalis and nervosa. Pars distalis revealed cells with different shapes, sizes, nuclei, and variable profiles of the cytoplasm. Pars nervosa, on the other hand, revealed pituicytes with electron-lucent cytoplasm and small apoptotic nuclei. Administration of ADSCs greatly improved the microscopic appearance of cells, while the recovery group showed similar histological changes as the fluoxetine group. Conclusion: Fluoxetine caused various deleterious changes in the pituitary gland of albino rats, as evidenced by electron microscopy. These changes were almost corrected by the ADSCs treatment. .

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慢性氟西汀对成年雄性白化大鼠垂体的影响及脂肪间充质干细胞可能的治疗作用的电镜研究

摘要背景：脂肪来源的间充质干细胞（ADSCs）具有自我更新和多潜能分化能力，在治疗各种疾病方面具有潜在的治疗潜力。工作目的：本研究旨在证明慢性氟西汀治疗后垂体的电镜结构以及ADSCs可能的治疗效果。材料与方法：将30只健康成年雄性白化大鼠分为对照组（Ⅰ组）。氟西汀治疗组（II组）接受溶于1.0mL自来水中的氟西汀24mg/kg/天，每天一次。用ADSCs治疗的氟西汀组（III组）作为组（II）接受氟西汀治疗30天，然后用ADSCs在尾静脉注射1次，剂量为1×106个细胞/大鼠，悬浮在0.5ml磷酸盐缓冲盐水（PBS）中。康复组（IV组）给予氟西汀治疗30天，直至实验结束。结果：氟西汀治疗组的超微结构观察显示，远端部和神经部均有明显的组织学变化。远端Pars显示细胞具有不同的形状、大小、细胞核和不同的细胞质轮廓。另一方面，神经膜显示垂体细胞具有电子透明的细胞质和小的凋亡细胞核。ADSCs的给药大大改善了细胞的微观外观，而恢复组表现出与氟西汀组相似的组织学变化。结论：氟西汀对白化病大鼠垂体产生了多种有害影响，电镜下可见。ADSCs治疗几乎纠正了这些变化。

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来源期刊

Ultrastructural Pathology 医学-病理学

CiteScore

2.00

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.